中国动物传染病学报2023，Vol.31Issue(5)：31-39,9.
实验性急性非洲猪瘟肝组织损伤及相关机制的研究
Study on Liver Tissue Injuries and Related Mechanisms of Experimental Acute African Swine Fever
摘要
Abstract
In order to explore the liver damages caused by African swine fever virus(ASFV)and its pathological mechanisms,acute African swine fever cases were reproduced in 13 pigs by inoculation of ASFV at the dose of 102 HAD50/mL(Pig/HLJ/18 strain).These pigs were terminated for observation of anatomical and histological changes of livers.The protein VP73 of ASFV in the livers was localized by in situ PCR.The expressions of inflammatory cytokines TNF-α,IL-1β,IFN-γ and apoptotic factors Bax and Bcl-2 in liver tissues were examined by immunohistochemical method.The results showed that ASFV caused obvious damages to the tissue structures of the livers and the pathological changes gradually aggravated with the prolongation of the course of disease.Liver congestion,hemorrhage,gray-white speckled inflammation,gallbladder edema and hyperemia were found at necropsy.The main histological lesions included hemorrhage,viral hepatitis,hepatocyte edema,necrosis,apoptosis and lymphocyte infiltration.ASFV was mainly distributed in hepatic lobular portal area,lobular interstitium,hepatic sinusoid,macrophages,hepatocytes and vascular endothelial cells.TNF-α,IL-1β and IFN-γ were mainly expressed in macrophages,hepatocytes,vascular endothelial cells and a few lymphocytes and their expression intensity increased significantly with the severity of injury(P<0.01).The expression of Bcl-2 decreased(P<0.01),which induced apoptosis.The results of this study showed that ASFV induced the release of inflammatory factors and activated the inflammatory cascade reaction through the destruction of intrahepatic monocyte-macrophage system,resulting in severe liver injury.关键词
非洲猪瘟/VP73/肝组织损伤/炎症因子/Bax/Bcl-2/病理机制Key words
African swine fever/VP73/liver injury/inflammatory cytokines/Bax/Bcl-2/pathological mechanism分类
畜牧业引用本文复制引用
李樵锋,邓桦,李慧,梁浩钊,徐志高,张伟伦,罗颖,许正涛,杨鸿,黄璐琦..实验性急性非洲猪瘟肝组织损伤及相关机制的研究[J].中国动物传染病学报,2023,31(5):31-39,9.基金项目
中央本级重大增减支项目(2060302) （2060302）
