中国动物传染病学报2023，Vol.31Issue(5)：71-78,8.
河南牛无乳链球菌耐药性分析和毒力基因检测
Analysis of Drug Resistance and Virulence Genes of Streptococcus agalactiae from Cattle in Henan
摘要
Abstract
In order to investigate the current status of drug resistance and distribution of virulence genes in Streptococcus agalactiae strains in Henan region,155 isolated strains were examined for drug susceptibility,resistance and virulence genes.The results showed that these 155 isolated strains were most susceptible to quinolone but resistant to tetracycline,aminoglycosides and macrolides.Especially,these strains were 100%resistant to penicillin G.The mutant penicillin resistance gene pbp2b were detected in all isolated strains.The aminoglycoside resistant genes aphA-3 and aad-6 were both contributed to the resistance of these strains.The resistance to tetracycline were mediated by tetL,tetM and tetK and their detection rates were 25.16%,31.61%and 19.35%respectively.The macrolide resistant genes erm(A/T)and ermB was also detected and their detection rates were 13.55%and 50.97%,respectively.In addition,the virulence genes bca,ScpB,hly and rib were also detected in these isolated strains and their detection rates were 48.39%,56.77%,20.65%and 23.87%,respectively.In summary,4 virulence genes and 8 drug resistance genes were distributed in S.agalactiae in Henan region,and multiple resistance genes were also found in most of those strains.关键词
奶牛乳房炎/无乳链球菌/耐药性/耐药基因/毒力基因Key words
Bovine mastitis/Streptococcus agalactiae/drug resistance/resistance gene/virulence gene分类
农业科技引用本文复制引用
张阳,胡云,李搏珍,李环,王志成,段丽莎,李生涛,史鸿飞..河南牛无乳链球菌耐药性分析和毒力基因检测[J].中国动物传染病学报,2023,31(5):71-78,8.基金项目
国家自然科学基金(31902263) （31902263）
河南省科技攻关项目(162102110046) （162102110046）
