中国动物传染病学报2023，Vol.31Issue(5)：79-86,8.
非洲猪瘟EP364R蛋白的原核表达及间接ELISA方法的建立
Expression of EP364R Protein of African Swine Fever Virus in Prokaryotic Cells and Development of an Indirect ELISA Detection Method
摘要
Abstract
The EP364R gene sequence of African Swine Fever Virus(ASFV)was synthesized according to the Georgia 2007/1 strain(GenBank accession number:FR682468.1)and amplified by PCR for insertion into pET-32a(+)vector.The resulting recombinant plasmid pET-32a-EP364R was verified and transformed into E.coli BL21(DE3)competent cells for protein expression.The purified recombinant protein was demonstrated in SDS-PAGE and Western blot.The purified recombinant protein was used as the coating antigen to develop an indirect ELISA method for detection of ASFV.The indirect ELISA method developed here was used to detect 84 clinical serum samples together with French ID-Vet African swine fever antibody kit.The agreement of these two methods reached to 89.3%,indicating that our indirect ELISA method had good specificity,sensitivity and repeatability.The availability of this new indirect ELISA method provided a rapid and practical tool for rapid diagnosis,epidemiological investigation and serological antibody detection of ASFV.关键词
非洲猪瘟/间接ELISA/EP364R/原核表达Key words
African swine fever virus/indirect ELISA/EP364R/prokaryotic expression分类
农业科技引用本文复制引用
卢丽飞,党佳佳,吴青萍,邹延林,奚媖牡,耿鑫梅,黄伟坚..非洲猪瘟EP364R蛋白的原核表达及间接ELISA方法的建立[J].中国动物传染病学报,2023,31(5):79-86,8.基金项目
广西创新驱动发展专项资金项目(桂科AA17204057-1) （桂科AA17204057-1）
广西自然科学基金(桂科AB16380135) （桂科AB16380135）
