中国动物传染病学报2023，Vol.31Issue(5)：108-113,6.
基于nuc基因的环介导等温核酸扩增可视化技术检测食源性金黄色葡萄球菌
Development of Loop Mediated Isothermal Amplification(Lamp)Visualization Technique Based on Nuc Gene for Detection of Foodborne Staphylococcus aureus
摘要
Abstract
Staphylococcus aureus food poisoning is mainly caused by the polluted foods with rich proteins or starch contents such as milks,meats,eggs,pastries and leftovers.Therefore,a rapid and accurate detection for S.aureus is particularly important for ensuring people's health and food safety.In this study,we developed a loop mediated isothermal nucleic acid amplification(LAMP)technique for the detection of S.aureus based on the nuc gene.The specificity of the LAMP detection showed that only the genomic DNA of S.aureus was positive,while those of other foodborne pathogens were negative,such as Listeria monocytogenes,Salmonella typhimurium,Enterohemorrhagic O157:H7,Campylobacter jejuni,Yersinia enterocolitica,Clostridium perfringens and swine origin pathogens such as Streptococcus suis,Actinobacillus pleuropneumoniae,Pasteurella multocida,suggesting that the LAMP developed in this study had good specificity.The detection limit of LMAP was 5.6×101 CFU/ml while that of the conventional PCR was 5.6×106 CFU/mL,indicating that the sensitivity of LAMP detection was 100 000 times higher than that of the conventional PCR.In addition,S.aureus was detected from 4 out of 50 clinical pork samples by both LAMP and conventional PCR.In summary,this study provided a rapid,visual,specific,sensitive and low-cost LAMP detection method for foodborne S.aureus.关键词
金黄色葡萄球菌/nuc基因/环介导等温核酸扩增技术/可视化Key words
Staphylococcus aureus/nuc gene/loop-mediated isothermal amplification(LAMP)/visual分类
轻工纺织引用本文复制引用
邹作成,刘浩洋,嵇辛勤,胡青海,王西,刘雪兰,胡仲皓,田祥强,何晓花,杜晓莉,王佳玲,李柯..基于nuc基因的环介导等温核酸扩增可视化技术检测食源性金黄色葡萄球菌[J].中国动物传染病学报,2023,31(5):108-113,6.基金项目
上海市科技创新项目(农业领域)(21N31900900) （农业领域）
国家重点研发项目(2018YFD0500500) （2018YFD0500500）
