中国动物传染病学报2023，Vol.31Issue(5)：114-119,6.
基于hipO基因的环介导等温核酸扩增技术检测食源性空肠弯曲菌
Development of Loop-mediated Isothermal Amplification Based on HipO Gene for Detecting Foodborne Campylobacter jejunum
摘要
Abstract
Campylobacter jejuni is one of the common food-borne pathogenic bacteria of human bacterial gastroenteritis,which seriously harms health.It is of great significance to develop a rapid and accurate method for detection of C.jejuni.In this study,a visual and low-cost loop mediated isothermal amplification(LAMP)method based on hipO gene was developed for rapid detection of C.jejuni.The LAMP detection was positive using the genomic DNA of C.jejuni CJFX01 as template but negative for enterohemorrhagic Escherichia coli O157:H7,Salmonella typhimurium,Listeria monocytogenes,Yersinia enterocolitica,Staphylococcus aureus,Clostridium perfringens and Actinobacillus pleuropneumoniae,Haemophilus parasuis,Pasteurella multocida,Streptococcus suis,Erysipelas suis or Bordetella bronchiseptica,indicating that it had good specificity.The detection limit of LAMP for C.jejuni was 5.4×101 CFU/mL while the detection limit of conventional PCR based on F3/B3 primers was 5.4×106 CFU/mL,indicating that the sensitivity of LAMP for detecting C.jejuni was 100,000 times higher than that of conventional PCR.The LAMP developed in this study had the advantages of rapid,visual,low cost,good specificity and high sensitivity,which might be suitable for rapid detection in laboratories and grass-roots food safety supervision departments.关键词
空肠弯曲菌/环介导等温核酸扩增(LAMP)/hipO/食源性致病菌/快速检测Key words
Campylobacter jejuni/loop-mediated isothermal amplification(LAMP)/hipO/food-borne pathogenic bacteria/rapid detection分类
轻工纺织引用本文复制引用
田祥强,刘雪兰,嵇辛勤,胡青海,王西,邹作成,胡仲皓,杜晓莉,何晓花,王佳玲,李柯,刘浩洋..基于hipO基因的环介导等温核酸扩增技术检测食源性空肠弯曲菌[J].中国动物传染病学报,2023,31(5):114-119,6.基金项目
国家重点研发项目(2018YFD0500500) （2018YFD0500500）
