中国动物传染病学报2023，Vol.31Issue(5)：128-134,7.
五日热巴尔通体RPA检测方法的建立
Real-time Recombinase Polymerase Amplification Assay for Rapid and Specific Detection of Bartonella quintana
摘要
Abstract
Trench fever characterized by sudden onset with fever and headache is transmitted by a body louse harboring the causative agent Bartonella quintana.Rapid and sensitive detection of the causative pathogen is essential for timely targeted therapy.In this study,we developed a real-time recombinase polymerase amplification(RPA)assay for the fast detection of B.quintana.The detection limit was 101 copies/μL,which was the same as real-time RCR.There was no cross-reaction with other Bartonella species(Bartonella henselae,Bartonella tribocorum,Bartonella vinsonii,Bartonella washoensis and Bartonella grahamii).Therefore,it was concluded that the RPA method developed in the present study was efficient in detection of Bartonella quintana infection and could be used for clinical diagnosis of bartonellosis关键词
五日热巴尔通体/重组聚合酶扩增/特异性/灵敏性Key words
Bartonella quintana/real-time recombinase polymerase amplification/specificity/sensitivity分类
医药卫生引用本文复制引用
丁田耘,陈琦,蔡雨豪,王蒙,王春艳,张浩然,袁聪俐..五日热巴尔通体RPA检测方法的建立[J].中国动物传染病学报,2023,31(5):128-134,7.基金项目
国家自然科学基金(31972653) （31972653）
