中国动物传染病学报2023，Vol.31Issue(5)：181-187,7.
牦牛源蜡样芽胞杆菌的分离鉴定及耐药性分析
Isolation,Identification and Drug Resistance of Bacillus Cereus from Yaks
摘要
Abstract
In order to study the prevalence and drug resistance of Bacillus cereus from yaks,this experiment was carried out for bacterial morphology observation,16S rDNA PCR and sequence comparison,animal regression test and drug susceptibility analysis.The results showed that 135 bacterial strains of were isolated from 56 yak milk samples.Then these isolated strains were tested using 16S rDNA PCR and sequenced.As a result,42 strains were identified as B.cereus with the detection rate of 75%(42/56),Accounting for 31.11%(42/135)of the total isolated bacteria.In addition,the 16S rDNA homology between the isolated strain B2019 with B.cereus in GenBank was 88.6%-99.3%.The 16S rDNA sequence of the isolated strain B2019 and 6 reference strains were compared with Mega7.0 for phylogenetic analysis.In the animal regression test,the inoculated mice had diarrhea.The autopsy of the infected mice found enlarged livers and bleeding lungs on the edges.Drug susceptibility testing showed that the isolated strains were highly susceptible to 13 antibiotics including mideacin,clindamycin,erythromycin,gentamicin,kanamycin,amikacin,neomycin,vancomycin,ofloxacin,ciprofloxacin,furazolidone and minocycline,medium susceptible to polymyxin B and resistant to the other 16 antibiotics.The test results showed that the isolated strain belonged to strongly pathogenic B.cereus.关键词
蜡样芽孢杆菌/分离鉴定/16S rDNA/耐药性Key words
Bacillus cereus/isolation and identification/16S rDNA/drug resistance分类
农业科技引用本文复制引用
王俊书,朱杰,郭宏元,徐进强,杨晶晶,金红岩..牦牛源蜡样芽胞杆菌的分离鉴定及耐药性分析[J].中国动物传染病学报,2023,31(5):181-187,7.基金项目
2017年西藏自治区科研创新团队项目 （）
2019年西藏自治区科技厅项目(XZ2019ZRG-149) （XZ2019ZRG-149）
