北京地区猫4种肠道原虫流行病学调查OA

Protozoa such as Cryptosporidium spp.and Giardia spp.,which can parasitize the cat's intestinal tracts,are known to cause infections in humans.In order to understand the infection status of common intestinal protozoa in cats in Beijing,this survey collected 628 fecal samples from cats in different regions of Beijing from June to December 2021.The nucleic acid was extracted,and PCR amplification and sequencing were performed on the ITS1-5.8S rRNA-ITS2 gene of Tritrichomonas foetus,small subunit ribosomal ribonucleic acid(SSU rRNA)gene of Giardia duodenalis,SSU rRNA gene of Cryptosporidium spp.,and cytochrome C oxidase subunit 1(mtCOI)gene of Cystoisospora spp.,and the infection rates and risk factors of these four intestinal protozoa were analyzed.For positive samples of Giardia duodenalis and Cryptosporidium spp.,molecular typing was performed based on the β-giardin(bg),glutamate dehydrogenase(gdh),and triosephosphate isomerase(tpi)genes of Giardia duodenalis and the glycoprotein 60(gp60)gene of Cryptosporidium spp..The results showed that the overall infection rate of intestinal protozoa in cats in Beijing was 20.22％(127/628),and the mixed infection rate was 16.54％(21/127).Among them,Tritrichomonas foetus had an infection rate of 10.19％(64/628),Giardia duodenalis had an infection rate of 6.69％(42/628),Cryptosporidium spp.had an infection rate of 4.46％(28/628),and Cystoisospora spp.had an infection rate of 3.03％(19/628).The genotypes of Giardia duodenalis were as follows:assemblage B(26/41),F(12/41),C(1/41),A Ⅰ(1/41),and A/F(1/41),while Cryptosporidium spp.were all of the Cryptosporidium felis,including subtype families ⅩⅨa(23/25)and ⅩⅨc(2/25).The analysis showed that purebred cats were more susceptible to Tritrichomonas foetus and Giardia duodenalis(P＜0.05),and the main clinical symptoms in infected cats were diarrhea or soft stools(P＜0.01).Cats under 1 year old,females,and non-sterilized cats were more susceptible to Cryptosporidium spp.(P＜0.05 or P＜0.01),while cats under 1 year old,non-sterilized,non-immunized,and not dewormed were more susceptible to Cystoisospora spp.(P＜0.01).The results indicate the presence of zoonotic genotypes in protozoa infecting cats in Beijing,including Giardia duodenalis assemblages A and B and Cryptosporidium spp.subtype families ⅩⅨa and ⅩⅨc,posing a potential risk of zoonotic transmission.