中国兽医杂志2024，Vol.60Issue(1)：52-58,7.
北京某绿地蚊媒病毒的分离和鉴定
Isolation and Identification of Mosquito-borne Viruses at a Park in Beijing
摘要
Abstract
In order to understand the distribution of mosquito-borne viruses in a park in Beijing,this study collected over 19 000 mosquito specimens using mosquito trap lamps.Virus isolation was performed using mosquito cells C6/36,and the isolated strains were identified using high-throughput sequencing and reverse transcription-polymerase chain reaction(RT-PCR).The viral sequences of the isolated strains were determined by RT-PCR and compared with corresponding viral sequences from domestic and international sources.The results showed that two strains causing significant cytopathic effects were obtained through virus isolation,named BJ2021/01 and BJ2021/02.High-throughput sequencing and RT-PCR results indicated that the BJ2021/01 strain contained a mixture of Banna virus(BAV),Shinobi tetravirus(SHTV),Sarawak virus(SWKV),and Culex originated tymoviridae-like virus(CuTLV).The BJ2021/02 strain was identified as BAV.Sequence analysis revealed that the nucleotide homology of the 12 segments of the BAV BJ2021/02 strain with other domestic and international BAV strains ranged from 58.1％to 98.7％,and the amino acid homology ranged from 54.4％to 98.9％.Phylogenetic analysis indicated that the BJ2021/02 strain belonged to the BAV genotype A.These results enrich the genomic sequences of BAV in China,providing a reference for epidemiological research on BAV in Beijing and offering a pathogenic basis for the prevention and control of mosquito-borne viral diseases.关键词
蚊媒病毒/版纳病毒/忍者病毒/沙捞越病毒/库蚊源类芜菁黄花叶病毒科病毒Key words
mosquito-borne virus/Banna virus/Shinobi tetravirus/Sarawak virus/Culex originated tymoviridae-like virus分类
农业科技引用本文复制引用
李金鑫,廖佳玮,张越,任行,任志浩,施霁桢,毕玉海,谭丽琼,苏敬良..北京某绿地蚊媒病毒的分离和鉴定[J].中国兽医杂志,2024,60(1):52-58,7.基金项目
北京圆明园遗址保护基金会资助[圆(服)字[2020]第178号] （服）
