北京某绿地蚊媒病毒的分离和鉴定OA

In order to understand the distribution of mosquito-borne viruses in a park in Beijing,this study collected over 19 000 mosquito specimens using mosquito trap lamps.Virus isolation was performed using mosquito cells C6/36,and the isolated strains were identified using high-throughput sequencing and reverse transcription-polymerase chain reaction(RT-PCR).The viral sequences of the isolated strains were determined by RT-PCR and compared with corresponding viral sequences from domestic and international sources.The results showed that two strains causing significant cytopathic effects were obtained through virus isolation,named BJ2021/01 and BJ2021/02.High-throughput sequencing and RT-PCR results indicated that the BJ2021/01 strain contained a mixture of Banna virus(BAV),Shinobi tetravirus(SHTV),Sarawak virus(SWKV),and Culex originated tymoviridae-like virus(CuTLV).The BJ2021/02 strain was identified as BAV.Sequence analysis revealed that the nucleotide homology of the 12 segments of the BAV BJ2021/02 strain with other domestic and international BAV strains ranged from 58.1％to 98.7％,and the amino acid homology ranged from 54.4％to 98.9％.Phylogenetic analysis indicated that the BJ2021/02 strain belonged to the BAV genotype A.These results enrich the genomic sequences of BAV in China,providing a reference for epidemiological research on BAV in Beijing and offering a pathogenic basis for the prevention and control of mosquito-borne viral diseases.