中国兽医杂志2024，Vol.60Issue(1)：59-65,7.
红螯螯虾源布氏柠檬酸杆菌分离鉴定和耐药性分析
Isolation,Identification and Drug Resistance Analysis of Citrobacter braakii from Cherax quadricarinatus
摘要
Abstract
In order to identify potential pathogens in Cherax quadricarinatus from a farm in Wuxu Town,Guangxi Zhuang Autonomous Region,China,a dominant strain named 171215-10 was isolated from the crayfish intestines in January 2021.Identification and analysis of the isolated strain were conducted using morphological observation,biochemical identification,construction of phylogenetic tree based on 16S rDNA sequence,bacterial growth curve determination,artificial infection of zebrafish,and Kirby-Bauer(K-B)disk diffusion method.The results showed that the identified isolated strain was Citrobacter braakii,which exhibited exponential growth from 5 to 16 hours.The artificial infection experiment on zebrafish demonstrated that the isolated strain was highly pathogenic,zebrafish injected with a concentration of 1.5 × 108 CFU/mL of Citrobacter braakii died within 2 days.Drug sensitivity testing revealed that the isolated strain was sensitive to only 5 out of 25 antibacterial drugs,including norfloxacin(NFX),chloramphenicol(CLM),doxycycline(DOX),amikacin(AMK),and gentamicin(GEN).The results of this study provide reference information for the identification,diagnosis,and treatment of Citrobacter braakii from Cherax quadricarinatus.关键词
布氏柠檬酸杆菌/红螯螯虾/分离鉴定/人工感染试验/药敏试验Key words
Citrobacter braakii/Cherax quadricarinatus/isolation and identification/artificial infection experiment/drug sensitivity test分类
医药卫生引用本文复制引用
黄亚明,李长乐,覃诗君,陆唯,张博洲,孙佳艺,张文旗,童潼,张琴..红螯螯虾源布氏柠檬酸杆菌分离鉴定和耐药性分析[J].中国兽医杂志,2024,60(1):59-65,7.基金项目
广西民族大学引进人才科研启动项目(2018KJQD14) （2018KJQD14）
广西创新驱动发展专项资金项目(桂科AA17204044) （桂科AA17204044）
广西民族大学相思湖青年学者创新团队资助项目(2018RSCXSHQN02) （2018RSCXSHQN02）
大学生创新训练项目(S202210608159) （S202210608159）
