中国兽医杂志2024，Vol.60Issue(1)：134-143,10.
复方中草药对兔生长性能和肠道健康的影响
Effects of Compound Chinese Herbal Medicine on Growth Performance and Intestinal Health in Rabbits
摘要
Abstract
To investigate the effects of compound Chinese herbal medicine on the growth performance,immune function,intestinal digestive enzyme activity,antioxidant capacity,intestinal morphology,and intestinal microbiota of rabbits,72 healthy 5-week-old New Zealand White rabbits were randomly divided into Control group(CN,fed with basic feed)and Chinese herbal medicine group(CM,basic feed supplemented with 600 mg/kg of compound Chinese herbal medicine)with 36 rabbits in each group.The experimental period lasted for 42 days.Feed intake and body weight gain were measured weekly,and average daily feed intake(ADFI),average daily gain(ADG),and feed-to-gain ratio(F/G)were calculated.On days 21 and 42 of the experiment,four healthy rabbits were randomly selected from each group,and blood was collected to detect biochemical and immune indicators.At the end of the experiment(day 42),the small intestine segments were collected,enzyme-linked immunosorbent assay(ELISA)was used to measure the enzyme activity and antioxidant indicators of the intestinal mucosa,hematoxylin-eosin(H.E.)staining was performed to observe intestinal villus morphology,quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the relative expression of tight junction protein mRNA,and high-throughput sequencing was employed to analyze cecal microbial diversity.The results showed no significant differences(P＞0.05)in ADFI,ADG,and F/G between the two groups.Compared with the CN group,on day 21 of the experiment,the serum total cholesterol(Tchol)content in the CM group was significantly decreased(P＜0.01).On day 42,the CM group exhibited a significant increase in serum immunoglobulin M(IgM)contents and secretory immunoglobulin A(sIgA)contents in the ileal mucosa(P＜0.05).Additionally,there was a significant increase in intestinal digestive enzyme activities(α-amylase,trypsin,and chymotrypsin)(P＜0.05);increased superoxide dismutase(SOD)activity in the jejunum(P＜0.05),decreased glutathione peroxidase(GSH-Px)activity(P＜0.05),and significantly reduced malondialdehyde(MDA)contents in the duodenum and jejunum(P＜0.05).Furthermore,the CM group exhibited a significant increase in villus length(V)in the jejunum(P＜0.05),a significant decrease in crypt depth(C)(P＜0.05),and a highly significant increase in the villus height to crypt depth ratio(V/C)(P＜0.01).The relative expression of occludin mRNA significantly upregulated in the duodenum(P＜0.05),and the relative expression of zona occludens-1(ZO-1)and occludin mRNA in the ileum also significantly upregulated(P＜0.05).Moreover,there was a significant increase in the richness index(Chao1)of bacterial communities in cecal contents(P＜0.05),with main dominant bacterial genera at the genus level of Clostridiales and Akkermansia.In conclusion,the addition of compound Chinese herbal medicine to rabbit diets enhanced immunity and antioxidant capacity,maintained the integrity of the intestinal mucosa,increased intestinal digestive enzyme activity,and improved intestinal microbiota.关键词
复方中草药/兔/生长性能/消化酶/肠道发育/肠道微生物Key words
compound Chinese herbal medicine/rabbit/growth performance/digestive enzyme/intestinal development/intestinal microbiota分类
农业科技引用本文复制引用
徐翔飞,黄盼,崔雪梅,季权安,肖琛闻,韦强,黄叶娥,鲍国连,刘燕..复方中草药对兔生长性能和肠道健康的影响[J].中国兽医杂志,2024,60(1):134-143,10.基金项目
浙江省"领雁"研发攻关计划项目(2023C02047) （2023C02047）
浙江省重点研发计划项目(2021C02007,2021C02068-7) （2021C02007,2021C02068-7）
国家现代农业产业技术体系建设专项基金(CARS-43-C-2) （CARS-43-C-2）
