中国兽医杂志2024，Vol.60Issue(1)：144-150,7.
HPLC-ELSD同时测定腺梗豨莶草中6个二萜成分的含量
Simultaneous Determination of Six Diterpenoids in Siegesbeckia pubescens Makino by HPLC-ELSD
摘要
Abstract
In order to study the contents of six diterpenoids(ent-pimarane:kirenol,hythiemoside B,and darutigenol;ent-kaurane:ent-17,18-dihydroxy-kauran-19-oic acid,ent-16β,17-dihydroxy-kauran-19-oic acid,and ent-16α-hydro-kauran-17,19-dioic acid)in Siegesbeckia pubescens Makino,this study established high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)for simultaneous determination of diterpenoids.The samples were pulverized,sieved,and reflux-extracted with methanol and ethyl acetate.After removing the solvent by vacuum evaporation,the residue was dissolved in methanol,filtered through 0.45 μm membrane,and the filtrate was determined.Chromatographic conditions:Waters Symmetry Shield™ RP18 column(250 mm ×4.6 mm,5 μm);mobile phase of 0.3％formic acid water solution-acetonitrile(v/v),gradient elution,flow rate of 1.0 mL/min.The ELSD drift tube temperature was set at 103℃,and the nebulizing gas flow rate was 3.0 L/min.This method was applied to determine the content of the six diterpenoids in different parts of Siegesbeckia pubescens Makino samples.Simultaneously,the differences in ent-pimarane,ent-kaurane,and total diterpenoids in leaves,branches,and stems were compared.The results showed that the six diterpenoids had good linearity within their linear range(r≥0.999 2),and the intra-day and inter-day precision relative standard deviations(RSD)were less than 3.5％.The recoveries ranged from 96.5％to 101.5％,with RSD less than 2.3％.The content of diterpenoids varied greatly in different parts of Siegesbeckia pubescens Makino(leaves,branches,stems).The results indicate that the established HPLC-ELSD is simple,accurate,and reproducible,providing a reference for comprehensive quality evaluation of Siegesbeckia pubescens Makino medicinal materials and the selection of the optimal medicinal parts for clinical application.关键词
高效液相色谱法(HPLC)/蒸发光散射检测器(ELSD)/腺梗豨莶草/含量测定/质量控制Key words
high performance liquid chromatography(HPLC)/evaporative light scattering detector(ELSD)/Siegesbeckia pubescens Makino/content determination/quality control分类
医药卫生引用本文复制引用
殷玥,张璇,呼小娜..HPLC-ELSD同时测定腺梗豨莶草中6个二萜成分的含量[J].中国兽医杂志,2024,60(1):144-150,7.基金项目
吉林省职业技术教育协会项目(2020XHZ020) （2020XHZ020）
长春医学高等专科学校校级课题 （）
