中国循证儿科杂志2023，Vol.18Issue(6)：452-455,4.DOI:10.3969/j.issn.1673-5501.2023.06.008
基于高通量测序对中国南北方地区学龄前健康儿童肠道菌群的横断面调查
Intestinal microflora diversity in healthy preschool child aged 3-7 in northern and southern China based on high-throughput sequencing:A cross-sectional survey
摘要
Abstract
Background Intestinal microbiota can affect human health through various ways and is related to the occurrence of diseases.There are obvious differences in the composition of intestinal microbiota in different regions.Objective To compare the difference of intestinal microbial composition among healthy preschool child in northern and southern China by 16S rRNA gene sequencing method.Methods Children aged 3-7 years who underwent physical examination at the physical examination center of Foshan Chancheng Hospital in Foshan City,Guangdong Province were selected as the southern group,and children from a kindergarten in Tacheng District of Xinjiang Uygur Autonomous Region were selected as the northern group.Stool samples of all preschool children were collected by their parents after training,and intestinal flora detection and related data analysis were completed by Hangzhou Guhe Information Technology Co.,LTD.Main outcome measures Microbial diversity,as well as differences in species composition.Results From February 1,2023 to April 1,2023,45 children aged(4.0±1.5)years were included in the southern group.In the northern group,45 cases were included,of which 2 were unqualified,and the remaining 43 were(4.1±1.3)years old.There were no significant differences in gender,age and ethnic composition between the two groups.The Alpha diversity indexes including Shannon,Simpson,Chao and ACE of the northern group were higher than those of the southern group with statistically significant differences between two groups.The weighted unifrac PCoA plot showed a significant separation between the two group samples.The abundance of Firmicutes in the northern group was higher than that in the southern group(55.1％vs 39.2％),and the abundance of Proteobacteria in the southern group was higher than that in the northern group(18.6％vs 7.2％).The abundance of Eubacterium-eligens-group in southern group was higher than that in northern group(11.8％vs 2.2％),and the abundance of Faecalibacterium in the northern group was higher than that in the southern group(13.9％vs 6.3％).The differences were statistically significant.LEfSe analysis and random forest intergroup prediction revealed that statistically different biomarkers were Escherichia-Shigella and Ruminococcus-gnavus group for the southern group and Faecalibacterium,Agathobacter,Lachnospira,Senegalimassilia,Subdoligranulum,Eubacterium-eligens-group,Parasutterella,Dialiste and Rseburia for the northern group.Conclusion There are significant differences in the intestinal flora of preschool children in the north and south of China.The regionality of the host should be fully considered in the future research on intestinal flora and diseases.关键词
肠道菌群/地域/学龄前儿童Key words
Intestinal microbes/Region/Preschool child引用本文复制引用
董文迪,金霞霞,莫珊,姜美琳,刘艳欢..基于高通量测序对中国南北方地区学龄前健康儿童肠道菌群的横断面调查[J].中国循证儿科杂志,2023,18(6):452-455,4.基金项目
佛山市莫珊名中医传承工作室建设项目:佛卫函[2020]92号 （）
佛山市"十四五"中医特色专科项目:佛卫函[2022]15号 （）
佛山市中医药骨干人才培养对象项目:佛卫函[2020]222号 （）
