Highly Aligned Ternary Nanofiber Matrices Loaded with MXene Expedite Regeneration of Volumetric Muscle LossOA北大核心CSTPCD
Current therapeutic approaches for volumetric muscle loss(VML)face challenges due to limited graft availability and insufficient bioactivities.To overcome these limitations,tissue-engineered scaffolds have emerged as a promising alternative.In this study,we developed aligned ternary nanofibrous matrices comprised of poly(lactide-co-ε-caprolactone)integrated with collagen and Ti_(3)C_(2)T_(x)MXene nanoparticles(NPs)(PCM matrices),and explored their myogenic potential for skeletal muscle tissue regeneration.The PCM matrices demonstrated favorable physicochemical properties,including structural uniformity,alignment,microporosity,and hydrophilicity.In vitro assays revealed that the PCM matrices promoted cellular behaviors and myogenic differentiation of C2C12 myoblasts.Moreover,in vivo experiments demonstrated enhanced muscle remodeling and recovery in mice treated with PCM matrices following VML injury.Mechanistic insights from next-generation sequencing revealed that MXene NPs facilitated protein and ion availability within PCM matrices,leading to elevated intracellular Ca^(2+)levels in myoblasts through the activation of inducible nitric oxide synthase(i NOS)and serum/glucocorticoid regulated kinase 1(SGK1),ultimately promoting myogenic differentiation via the m TOR-AKT pathway.Additionally,upregulated i NOS and increased NO–contributed to myoblast proliferation and fiber fusion,thereby facilitating overall myoblast maturation.These findings underscore the potential of MXene NPs loaded within highly aligned matrices as therapeutic agents to promote skeletal muscle tissue recovery.
Moon Sung Kang;Yeuni Yu;Rowoon Park;Hye Jin Heo;Seok Hyun Lee;Suck Won Hong;Yun Hak Kim;Dong‑Wook Han;
Department of Cogno‑Mechatronics Engineering,College of Nanoscience and Nanotechnology,Pusan National University,Busan 46241,Republic of KoreaMedical Research Institute,School of Medicine,Pusan National University,Yangsan 50612,Republic of KoreaDepartment of Anatomy,School of Medicine,Pusan National University,Yangsan 50612,Republic of KoreaDepartment of Cogno‑Mechatronics Engineering,College of Nanoscience and Nanotechnology,Pusan National University,Busan 46241,Republic of Korea Present Address:Osstem Implant Inc.,Seoul 07789,Republic of KoreaDepartment of Cogno‑Mechatronics Engineering,College of Nanoscience and Nanotechnology,Pusan National University,Busan 46241,Republic of Korea Engineering Research Center for Color‑Modulated Extra‑Sensory Perception Technology,Pusan National University,Busan 46241,Republic of KoreaMedical Research Institute,School of Medicine,Pusan National University,Yangsan 50612,Republic of Korea Department of Biomedical Informatics,School of Medicine,Pusan National University,Yangsan 50612,Republic of Korea Periodontal Disease Signaling Network Research Center and Dental and Life Science Institute,School of Dentistry,Pusan National University,Yangsan 50612,Republic of KoreaDepartment of Cogno‑Mechatronics Engineering,College of Nanoscience and Nanotechnology,Pusan National University,Busan 46241,Republic of Korea BIO‑IT Fusion Technology Research Institute,Pusan National University,Busan 46241,Republic of Korea
Ti_(3)C_(2)T_(x)MXene nanoparticleTernary nanofibrous matricesMyogenesisRegeneration of volumetric muscle lossNext generation sequencing
《Nano-Micro Letters》 2024 (004)
P.269-292 / 24
supported by the National Research Foundation of Korea(NRF)grant funded by the Korean Government(the Ministry of Science and ICT(MSIT))(No.2021R1A2C2006013);the Bio&Medical Technology Development Program of the NRF funded by the Korean government(MSIT)(No.RS-2023-00223591);the Korea Medical Device Development Fund grant funded by the Korean government(the MSIT,the MOTIE,the Ministry of Health and Welfare,the Ministry of Food and Drug Safety)(NTIS Number:9991006781,KMDF_PR_(2)0200901_0108)。
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