北京中医药大学学报2024，Vol.47Issue(1)：9-15,7.DOI:10.3969/j.issn.1006-2157.2024.01.003
益肾达络饮对复发缓解型多发性硬化患者缺氧诱导因子-1α和细胞因子表达的影响
Effects of Yishen Daluo Decoction on the serum levels of the glycolysis-related protein HIF-1α and cytokines in patients with relapsing-remitting multiple sclerosis
摘要
Abstract
Objective We aimed to assess the serum levels of the glycolysis-related protein hypoxia-inducible factor-1α(HIF-1α)and cytokines in patients with relapsing-remitting multiple sclerosis(RRMS)before and after treatment with the traditional Chinese medicine Yishen Daluo Decoction,as well as their correlation with clinical parameters,and explore the immune regulatory mechanism of Yishen Daluo Decoction on multiple sclerosis.Methods Twenty-eight patients with RRMS in remission recruited from May 2018 to January 2022 in the Multiple Sclerosis Clinic at Dongzhimen Hospital,Beijing University of Chinese Medicine were retrospectively included.Comparisons were made with paired samples from 13 of them before and after Yishen Daluo Decoction treatment.A total of 20 gender-and age-matched healthy controls(HCs)were also recruited.Clinical information was collected from all of the subjects,and the neurological impairments of RRMS patients were assessed with the Expanded Disability Status Scale(EDSS)score.ELISA and SP-X multiplex cytokine assays were used to measure the serum levels of HIF-1 α and 10 cytokines(IL-1β,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12p70,IL-22,IFN-γ,and TNF-α),respectively.Spearman's method was used to analyze the correlation between the serum levels of HIF-1α and cytokines,and the correlation between the serum levels of HIF-1α and cytokines and patients'clinical indicators,including disease duration,the number of attacks,and the EDSS score.Results Serum HIF-1α,IL-4,IL-6,IL-8,IL-10,IL-12p70,IFN-γ,and TNF-α levels in the RRMS group were significantly higher than those in the HC group(P＜0.05).Serum levels of HIF-1α,IL-4,IL-6,and IL-12p70 in the Yishen Daluo Decoction-pre group were significantly higher than those in the Yishen Daluo Decoction-post group(P＜0.05).In addition,the serum HIF-1α level was positively correlated with IL-6(r=0.452,P=0.016)and TNF-α(r=0.524,P=0.004)levels in patients with RRMS.The IFN-γ level was negatively correlated with the EDSS score(r=-0.423,P=0.025),the IL-4 level was negatively correlated with disease duration(r=-0.385,P=0.043),and the TNF-α level was positively correlated with disease duration(r=0.397,P=0.037).Conclusion The regulatory mechanism of Yishen Daluo Decoction on immune imbalance in RRMS may be related to its ability to reduce the serum levels of the glycolysis-related protein HIF-1α in RRMS patients.It is also related to the levels of various inflammatory cytokines.关键词
益肾达络饮/多发性硬化/缺氧诱导因子-1α/糖酵解相关蛋白/细胞因子Key words
Yishen Daluo Decoction/multiple sclerosis/hypoxia-inducible factor-1α/glycolysis-related protein/cytokine分类
医药卫生引用本文复制引用
刘佳,关晓睿,张靖泽,杨琦,芮一峰,高颖..益肾达络饮对复发缓解型多发性硬化患者缺氧诱导因子-1α和细胞因子表达的影响[J].北京中医药大学学报,2024,47(1):9-15,7.基金项目
国家自然科学基金项目(No.82205034) （No.82205034）
中央高校基本科研业务费专项资金项目(No.2023-JYB-JBQN-017) （No.2023-JYB-JBQN-017）
北京市自然科学基金项目(No.7222114) （No.7222114）
中医药传承与创新"百千万"人才工程(岐黄工程)项目(国中医药人教发[2018]12号)National Natural Science Foundation of China(No.82205034) （岐黄工程）
Fundamental Research Funds for the Central Universities(No.2023-JYB-JBQN-017) （No.2023-JYB-JBQN-017）
Beijing Natural Science Foundation(No.7222114) （No.7222114）
Chinese Medicine Inheritance and Innovation Talent Project Leading Talent Support Program of National Traditional Chinese Medicine(No.[2018]12) （No.[2018]12）
