北京中医药大学学报2024，Vol.47Issue(1)：70-80,11.DOI:10.3969/j.issn.1006-2157.2024.01.011
基于OPG/RANKL/RANK信号通路探讨桂枝芍药知母汤对胶原诱导性关节炎大鼠的影响
Effect of Guizhi Shaoyao Zhimu Decoction on collagen-induced arthritis rat based on OPG/RANKL/RANK signaling pathway
摘要
Abstract
Objective To study the effects of Guizhi Shaoyao Zhimu Decoction on factors related to bone destruction and bone protection in rats with collagen-induced arthritis(CIA)based on osteopro-tegerin(OPG)/receptor activator of NF-κB ligand(RANKL)/receptor activator of NF-κB(RANK)signaling pathway.Methods According to the body weight,60 female Wistar rats were randomly di-vided into the following six groups:the normal group,the model group,the Triperygium wilfordii mul-tiglucoside group(0.01 g/kg),the Guizhi Shaoyao Zhimu Decoction low-dose group(8.6 g/kg),the Guizhi Shaoyao Zhimu Decoction medium-dose group(17.2 g/kg),and the Guizhi Shaoyao Zhimu Decoction high-dose group(34.4 g/kg)(n=10 rats per group).The rats in all groups except for the normal group were given 100 μg bovine type Ⅱ collagen on the 1st and 8th days to establish the CIA model,and was injected into the left foot sole and tail root of the rats.After the successful modeling,the rats were treated by gavage for 4 weeks.The general state,body weight,and arthritis index(AI)score of rats were recorded,and the contents of RANKL and OPG in rat serum were determined by enzyme-linked immunosorbent assay.The mRNA and protein expressions of RANKL,RANK,and OPG in the ankle joint were determined through real-time PCR and Western blotting,respectively.Results Com-pared with the normal group,the general state of the model group was poor,the toe swelling was obvious,the AI score was increased,the serum RANKL content was increased,the serum OPG content was de-creased,and the mRNA and protein expressions of RANKL and RANK in the ankle joint were increased(P＜0.05).Compared with the model group,the degree of toe swelling and the AI score of rats in the Guizhi Shaoyao Zhimu Decoction low-,medium-,and high-dose groups were decreased,the serum RANKL content was decreased,the serum OPG content was increased,the mRNA and protein expres-sions of RANKL and RANK in the ankle joint were decreased,the mRNA and protein expressions of OPG were increased,and the RANKL/OPG ratio of the ankle joint was decreased(P＜0.05).Conclusion Guizhi Shaoyao Zhimu Decoction can improve the destruction of joint bone in CIA rats,and its mecha-nism of action may be related to reducing RANKL level,reducing RANKL/OPG ratio,and regulating bone balance.关键词
桂枝芍药知母汤/护骨因子/NF-κB受体激活蛋白配体/NF-κB受体激活蛋白信号通路/胶原诱导性关节炎/骨代谢/骨平衡/大鼠Key words
Guizhi Shaoyao Zhimu Decoction/OPG/RANKL/RANK signaling pathway/collagen-induced arthritis/bone metabolism/bone balance/rats分类
医药卫生引用本文复制引用
杨双庆,冼锦忠,马俊福,孟庆良,丁明辉,刘亚伟,张苏丹,王妍琦,李锦锦,李玉洁..基于OPG/RANKL/RANK信号通路探讨桂枝芍药知母汤对胶原诱导性关节炎大鼠的影响[J].北京中医药大学学报,2024,47(1):70-80,11.基金项目
国家自然科学基金项目(No.81804050) （No.81804050）
河南省中医药科学研究专项重大课题(No.20-21ZYZD05) （No.20-21ZYZD05）
河南省中医药科学研究专项普通课题(No.2022ZY1062) （No.2022ZY1062）
河南中医药大学2022年度研究生科研创新项目(No.2022KYCX085)National Natural Science Foundation of China(No.81804050) （No.2022KYCX085）
Special Major Subject of Sci-entific Research of Traditional Chinese Medicine in Henan Province(No.20-21ZYZD05) （No.20-21ZYZD05）
Special Gen-eral Subject of Scientific Research of Traditional Chinese Medicine in Henan Province(No.2022ZY1062) （No.2022ZY1062）
