北京中医药大学学报2024，Vol.47Issue(1)：124-132,9.DOI:10.3969/j.issn.1006-2157.2024.01.017
基于谷胱甘肽抗氧化系统探讨督脉电针对脑卒中后肢体痉挛大鼠氧化应激的影响
Effects of governor vessel electroacupuncture on oxidative stress in rats with post-stroke limb spasm based on the glutathione antioxidant system
摘要
Abstract
Objective To evaluate the mechanism of governor vessel electroacupuncture in rats with post-stroke limb spasm by observing the changes of glutathione antioxidant system-related factors.Methods A total of 60 SD rats were randomly divided into the normal group(n=12),sham operation group(n=12)and modeling group(n=36).The middle cerebral artery obstruction model was prepared by thread approach method in the modeling group,and 24 rats with successful modeling were randomly divided into the model group and the electroacupuncture group,with 12 rats in each group.At the 3rd day after modeling,the electroacupuncture group was treated with electroacupuncture at three acupoints of the governor vessel,namely,"Dazhui"(GV14),"Jizhong"(GV6)and"Houhui"(anteromedial of the transverse process of the sixth lumbar vertebra),for 30 min each time,once a day for 7 days.The neurological function of rats was assessed by Zea Longa neurological deficit score.The muscle tension of rats was detected by modified Ashworth dystonia rating and electrophysiological tracing method.The brain tissue water content was measured by the dry-wet weight method.The volume of cerebral infarction of rats was measured by the TTC staining method.The contents of glutathione(GSH),catalase(CAT),oxidized glutathione(GSSG),superoxide dismutase(SOD),and malondialdehyde(MDA)in the cortex of rats were detected by colorimetry.The protein and mRNA expressions of glutathione reductase(GR),glutamate cysteine ligase(GCL)C,GCLM,and glutathione peroxidase 4(GPX4)in the cortex of rats were measured by Western blotting and real-time PCR,respectively.Results Compared with rats in the normal and sham operation groups,the Zea Longa neurological deficit score,modified Ashworth dystonia rating,the volume of cerebral infarction,brain tissue water content,and GSSG and MDA contents in cortex were increased in the model group,the tension signal value and the proteins and mRNA expressions of GR,GCLC,GCLM,and GPX4 in cortex were decreased,and the contents of GSH,CAT,and SOD in cortex were decreased(P＜0.05).Compared with the model group,the Zea Longa neurological deficit score,modified Ashworth dystonia rating,the volume of cerebral infarction,brain tissue water content,and GSSG and MDA contents in cortex were decreased in the electroacupuncture group,the tension signal value and the proteins and mRNA expressions of GR,GCLC,GCLM,and GPX4 in cortex were increased,and the contents of GSH,CAT,and SOD in cortex were increased(P＜0.05).Conclusion Governor vessel electroacupuncture can improve the severity of post-stroke limb spasm in rats,and its mechanism may be related to the regulation of glutathione antioxidant system in cerebral cortex.关键词
脑卒中/肢体痉挛/督脉/电针/谷胱甘肽抗氧化系统/氧化应激/大鼠Key words
stroke/limb spasm/governor vessel/electroacupuncture/glutathione antioxidant system/oxidative stress/rats分类
医药卫生引用本文复制引用
张建云,梅紧紧,张帅迪,顾昌宇,李晶晶,郭健,冯晓东,李瑞青..基于谷胱甘肽抗氧化系统探讨督脉电针对脑卒中后肢体痉挛大鼠氧化应激的影响[J].北京中医药大学学报,2024,47(1):124-132,9.基金项目
国家自然科学基金青年基金项目(No.81503630) （No.81503630）
河南省重点研发与推广专项(科技攻关)(No.232102310470) （科技攻关）
省级科技研发计划联合基金(优势学科培育类)项目(No.222301420066) （优势学科培育类）
河南中医药大学第一附属医院国家中医药传承创新专项(No.2022CCCX010)National Natural Science Foundation of China(No.81503630) （No.2022CCCX010）
Henan Province Key R&D and Promotional Special Project(Scientific and Technological Research)(No.232102310470) （Scientific and Technological Research）
Provincial Science and Technology Research and Development Program Joint Fund(Advantageous Discipline Cultivation Category)Project(No.222301420066) （Advantageous Discipline Cultivation Category）
