华东理工大学学报（自然科学版）2024，Vol.50Issue(1)：1-3,3.DOI:10.14135/j.cnki.1006-3080.20240117001
卤代甲基转移酶的发现与应用研究进展
Discovery and Research Progress of Halide Methyltransferases
摘要
Abstract
In recent years,methylation reactions have garnered increasing attention in both organic and biosynthesis fields.By introducing methyl groups at appropriate positions in compound structures,the molecular chemical structure,physicochemical properties,and biological activity can be altered,imparting new functionalities.This facilitates the creation of more purpose-specific functional molecules.The enzymatic-catalyzed alkylation reactions in biocatalysis,known for their high chemical,regional,or stereo selectivity,have gained widespread attention and played a crucial role in the biosynthesis of natural products.The introduction of methyl groups through methyltransferases(MT)offers a novel avenue for precisely designing the structure and function of target molecules,thereby modulating their biological activity and physicochemical properties.Notably,halide methyltransferases(HMT),a unique subclass of MT,not only catalyze the generation of diverse halogenated hydrocarbons but also achieve enzymatic,in-situ regeneration of the expensive cofactor S-adenosylmethionine(SAM)in the presence of cost-effective,non-natural methyl donors like iodomethane.This review highlights the latest research advancements and breakthroughs in HMT,showcasing the development of a simple and versatile SAM recycling system through the introduction of HMT-MT cascade,thereby enhancing the atomic economy of reactions.The identification of enzymes within the thiopurine methyltransferase family(TPMT)addresses environmental concerns related to methyl donors.Furthermore,variants obtained through directed evolution optimize HMT,facilitating the transfer of longer chain alkyl groups.These innovative studies provide novel strategies for efficient biological alkylations,potentially revolutionizing green biomanufacturing technologies.关键词
卤代甲基转移酶/辅因子原位再生/S-腺苷甲硫氨酸/蛋白质工程/酶促级联反应Key words
halide methyltransferase/cofactor in-situ regeneration/S-adenosylmethionine/protein engineering/enzyme-catalyzed cascade reaction分类
生物科学引用本文复制引用
陈琦,张诗雨,高春玉,郑高伟,许建和..卤代甲基转移酶的发现与应用研究进展[J].华东理工大学学报（自然科学版）,2024,50(1):1-3,3.基金项目
国家重点研发计划(2021YFC2102300,2019YFA0905000) （2021YFC2102300,2019YFA0905000）
国家自然科学基金(31971380) （31971380）
上海市合成生物学重点专项(23HC1400200) （23HC1400200）
