扬州大学学报（农业与生命科学版）2023，Vol.44Issue(6)：103-108,6.DOI:10.16872/j.cnki.1671-4652.2023.06.013
绶草中活性成分Spiranthol-A的快速制备及抑制乙酰胆碱酯酶活性研究
Study on the rapid preparation and acetylcholinesterase-inhibitory activity of Spiranthol-A,an active compound from Spiranthes sinensis
摘要
Abstract
In order to establish a"macroporous adsorption resin column chromatography-gel column chromatography-HPLC"three-step rapid preparation method and evaluate the acetylcholinesterase(AChE)-inhibitory activity of Spiran-thol-A,an active compound in Spiranthes sinensis,the 95％ethanol extract of S.sinensis was separated by macroporous adsorption resin column chromatography to obtain the fraction containing Spiranthol-A,which was then purified by the Sephadex-LH 20 column chromatography and HPLC to get Spiranthol-A,guided by the HPLC.In addition,the in vitro AChE-inhibitory activity of Spiranthol-A was tested by a microplate assay.The results showed that Spiranthol-A obtained showed a high purity and a strong AChE-inhibitory activity,with an IC50 value of(10.58±0.44)μg·mL-1.The rapid preparation method established in this paper has a certain application prospect.Spiranthol-A has a significant AChE-in-hibitory effect and is worthy of a further study.关键词
绶草/Spiranthol-A/快速制备/结构鉴定/乙酰胆碱酯酶抑制活性Key words
Spiranthes sinensis/Spiranthol-A/rapid preparation/structural identification/AChE-inhibitory activity分类
农业科技引用本文复制引用
王建斌,高堑,何贵玲,邹美佳,刘量..绶草中活性成分Spiranthol-A的快速制备及抑制乙酰胆碱酯酶活性研究[J].扬州大学学报（农业与生命科学版）,2023,44(6):103-108,6.基金项目
江苏高校"青蓝工程"项目(20200104) （20200104）
扬州大学"青蓝工程"项目(20180210) （20180210）
扬州大学"高端人才支持计划"项目(20190223) （20190223）
扬州大学大学生学术科技创新基金项目(x20200763) （x20200763）
