军事医学2024，Vol.48Issue(1)：30-35,6.DOI:10.7644/j.issn.1674-9960.2024.01.006
高毒力肺炎克雷伯菌两种金黄地鼠呼吸道感染模型比较研究
Two golden hamster models of hypervirulent Klebsiella pneumoniae respiratory infection:a comparative study
摘要
Abstract
Objective To establish two golden hamster models infected with hypervirulent Klebsiella pneumoniae via aerosolized intratracheal(i.t.)and intranasal(i.n.)inoculation,and compare their properties.Methods Golden hamsters of 4 to 5 weeks old were exposed to K.pneumoniae NTUH-K2044 via i.t.route and i.n.route respectively.The survival of these golden hamsters was observed and recorded within 14 days of infection before the 50%lethal dose(LD50),survival rate,bacterial respiratory deposition rate,lung bacterial load and histopathology of the infected golden hamsters in the two groups were detected.Results The LD50 of the i.t.route(3×104 CFU)was lower than that of the i.n.route(7×105 CFU)in golden hamsters.After 4×106 CFU NTUH-K2044 infection,the golden hamsters in the i.t.group had 96.46%of the bacteria deposited and colonized in the lung,developed lobar pneumonia and died without exception within 4 days of infection,while those in the i.n.group had 95.62%of the bacteria deposited in the mouth and nose initially before the bacteria moved down to the trachea for colonization and were cleared out gradually.This group mainly acquired bronchopneumonia with relatively mild lung lesions,with a 14-day survival rate of 70%.Conclusion Inoculation routes can make a difference to the disease type of respiratory tract infections in animal models.The i.t.route mainly causes lobar pneumonia with severe lung lesions,while the i.n.route leads to bronchopneumonia with mild lung lesions.The two animal models established above may be utilized for pathogenesis investigation and treatment efficacy evaluation of Klebsiella pneumoniae.关键词
高毒力肺炎克雷伯菌/气溶胶/金黄地鼠/动物模型Key words
hypervirulent Klebsiella pneumoniae/aerosol/golden hamster/animal model分类
医药卫生引用本文复制引用
张之珺,张丽丽,吕蒙,周冬生,杨文慧,高波..高毒力肺炎克雷伯菌两种金黄地鼠呼吸道感染模型比较研究[J].军事医学,2024,48(1):30-35,6.基金项目
国家重点研发计划项目(2023YFC2605000) （2023YFC2605000）
病原微生物生物安全国家重点实验室自主研究课题(SKLPBS2225) （SKLPBS2225）
