军事医学2024，Vol.48Issue(1)：36-43,8.DOI:10.7644/j.issn.1674-9960.2024.01.007
去泛素化酶Mysm1通过调控胶质纤维酸性蛋白的表达调节神经干细胞向星形胶质细胞分化
Deubiquitinase Mysm1 regulates differentiation of neural stem cells into astrocytes by regulating expressions of glial fibrillary acidic protein
摘要
Abstract
Objective To investigate the effect of Mysm1 on the differentiation of neural stem cells(NSCs)into astrocytes and the possible mechanism.Methods NSCs were prepared from E12.5 cortices of wild-type C57BL/6 mice,cultured in vitro and induced to differentiate into astrocytes.Immunofluorescence staining,real-time quantitative PCR and Western blot assay were used to detect the expressions of Mysm1 during the differentiation of NSCs into astrocytes in vitro.Lentivirus was used to knock down Mysm1 expressions in NSCs before real-time quantitative PCR and Western blot assay were used to detect the knockdown efficiency.Immunofluorescence staining and Western blot assay were used to compare the differentiation of NSCs into astrocytes before and after Mysm1 knockdown in vitro.Transcriptomics was adopted to detect the differential gene after knockdown of Mysm1 in NSCs in vitro.Western blot assay was used to verify the changes of proteins associated with the differential gene.Cut-Tag was used to detect the enrichment of Mysm1 in the promoter region of glial fibrillary acidic protein(GFAP)genes during the differentiation of NSCs into astrocytes in vitro.After overexpression of GFAP following knockdown of Mysm1,immunofluorescence staining and Western blot assay were used to compare the differentiation of NSCs into astrocytes before and after overexpression in vitro.Results The expression of Mysm1 was gradually increased when NSCs were induced to differentiate into astrocytes in vitro.Mysm1 knockdown inhibited the differentiation of NSCs into astrocytes in vitro.Mysm1 affected the differentiation of NSCs into astrocytes by regulating the expression of GFAP.Overexpression of GFAP after Mysm1 knockdown partially rescued the ability of NSCs to differentiate into astrocytes.Conclusion Mysm1 regulates the differentiation of NSCs into astrocytes by epigenetically controlling GFAP transcription.关键词
Mysm1/神经干细胞/星形胶质细胞/分化Key words
Mysm1/neural stem cells/astrocytes/differentiation分类
医药卫生引用本文复制引用
徐振华,秦巧臻,李晓彤,陈悦,江小霞,徐东刚..去泛素化酶Mysm1通过调控胶质纤维酸性蛋白的表达调节神经干细胞向星形胶质细胞分化[J].军事医学,2024,48(1):36-43,8.基金项目
国家自然科学基金面上项目(31971285) （31971285）
后勤科研项目(145BHQ090003000X09,145BHQ090003000X13) （145BHQ090003000X09,145BHQ090003000X13）
