山羊CIDEa基因过表达对乳腺脂质合成的影响OA北大核心CSTPCD

Cell-death-inducing DNA fragmentation factor-α-like effector a(CIDEa)is a lipid droplet associated protein,which is involved in regulating lipid metabolism and maintaining body energy balance.In order to further explore the function of CIDEa in lipid metabolism in mammary gland of dairy goats(Capra hircus),in this study,dairy goat mammary epithelial cells were used as a model to study the effect of CIDEa gene on lipid synthesis by constructing pcDNA3.1-CIDEa overexpression vector.The results showed that the mRNA expression of CIDEa gene was increased nearly 10-fold after 48 h of transfection with pcDNA3.1-CIDEa in dairy goat mammary epithelial cells(P＜0.01).The results of triacylglycerol(TAG)contents assay and Oil Red O staining showed that the accumulation of lipid droplets and the content of triglyceride(P＜0.05)in cells were increased after CIDEa overexpression.The results of real-time quantitative PCR showed that CIDEa overexpression significantly increased the expression of acetyl-CoA carboxylase alpha(ACACA)(P＜0.05),cluster of differentiation 36(CD36)(P＜0.05),sterol regulatory element binding protein 1(SREBP1)(P＜0.05)and diacylglycerol acyltransferase 2(DGAT2)(P＜0.01).These results indicated that CIDEa gene could promote lipid synthesis in mammary epithelial cells.This study provides a theoretical basis for further studying the role of CIDEa gene in the regulation of milk fat metabolism in dairy goats.