农业生物技术学报2024，Vol.32Issue(3)：617-627,11.DOI:10.3969/j.issn.1674-7968.2024.03.012
斑点叉尾鮰伪雌鱼卵巢发育评估及比较转录组研究
Evaluation of Ovarian Development and Comparative Transcription Study of Channel Catfish(Ictalurus punctatus)Pseudofemale
摘要
Abstract
The cultivation of XY type pseudo female fish is a necessary step in obtaining faster growing all male strains of channel catfish(Ictalurus punctatus).In order to investigate the ovarian development at the period of adult and transcriptomic expression during sex differentiation after XY female channel catfish was induced by 17β-estradiol.The ovarian development and oocyte development of channel catfish were compared and analyzed by histological and anatomical methods with positive genetic sex identification,and the gonad development of XY pseudo-female channel catfish at the 3-year-old(500～2000 g)was evaluated by statistical gonad index.Transcriptomic sequencing was performed on XY pseudo-female channel catfish and normal female channel catfish at the age of 60 d after hatching.The gonad index of the 3-year-old XY female channel catfish was significantly different,the ovary of the large size XY females and the normal females was larger,with long sacs,densely filled with blood vessels and full of yolk.Oocytes in vitellogenic stage were mainly accompanied by cortical follicular stage and a little number of primary growth oocytes.Transcriptome sequencing revealed 11 288 differentially expressed genes between the ovaries of 60 d after hatching XY type pseudo female fish and normal female fish,enriched in multiple immune related biological processes and signaling pathways.According to GO enrichment and KEGG signaling pathway,among the top 30 GO enriched pathways,the female gamete generation pathway was screened,in which 32 genes showed differential expression,with down regulation of progesterone receptor(pgr)gene and androgen receptor(ar)gene expression.The research provides reproductive biology techniques and theoretical basis for sex control breeding of channel catfish.关键词
斑点叉尾鮰/雌性化/17β-雌二醇/转录组测序/性腺评估Key words
Channel catfish/Feminization/17β-estradiol/Transcriptome sequencing/Gonad evaluation分类
农业科技引用本文复制引用
徐思琪,张世勇,王明华,刘洪岩,钟立强,陈校辉..斑点叉尾鮰伪雌鱼卵巢发育评估及比较转录组研究[J].农业生物技术学报,2024,32(3):617-627,11.基金项目
国家现代农业产业技术体系"国家特色淡水鱼产业技术体系"(CARS-46) （CARS-46）
国家自然科学基金(32102771) （32102771）
江苏省农业重大新品种创制项目(PZCZ201741) （PZCZ201741）
江苏省自然科学基金(BK20191487) （BK20191487）
