农业生物技术学报2024，Vol.32Issue(3)：628-640,13.DOI:10.3969/j.issn.1674-7968.2024.03.013
近江蛏溶质载体6(SLC6)基因家族的鉴定及其在盐胁迫下的表达分析
Identification of Solute Carrier 6(SLC6)Gene Family of Sinonovacula rivularis and Its Expression Analysis Under Salinity Stress
摘要
Abstract
The solute carrier 6(SLC6)gene family,known as neurotransmitter transporters,plays a crucial role in stress regulation and osmotic regulation of aquatic organisms.In the present study,the members of the SLC6 gene family of Sinonovacula rivularis(SrSLC6)were identified and analyzed by using bioinformatics methods,and expression patterns under salinity stress were investigated.The results showed that a total of 29 SrSLC6 genes were identified,which belonged to 4 subfamilies and contained 12 conserved motifs and 2 conserved domains SLC6sbd-TauT-like and SLC5-6-like_sbd.Under high and low salinity stress,16 SrSLC6 gene family members showed different expression responses and their expression levels were up-regulated or down-regulated.Amino acid transporter 4 protein of S.rivularis(SrAAT4)related to salinity adaptation was mainly located in lateral ciliated columnar cells and flat cells on the side of gill filaments,and the expression patterns showed the same trend as transcriptome expression profile,which was significantly decreased at different time points under low salt stress(P＜0.05),and significantly increased at different time points under high salt stress(P＜0.05).These results indicated that the amino acid transporters(AATs)and neurotransmitter transporters(NTTs)members of SrSLC6 gene family might played important roles in osmotic regulation of S.rivularis,which provided theoretical basis for further research on the osmotic regulation and salinity adaptation of SLC6 gene family in mollusks.关键词
近江蛏/溶质载体6(SLC6)/基因家族/盐胁迫Key words
Sinonovacula rivularis/Solute carrier 6(SLC6)/Gene family/Salinity stress分类
农业科技引用本文复制引用
王莎莎,姚韩韩,何琳,何京,董迎辉,林志华..近江蛏溶质载体6(SLC6)基因家族的鉴定及其在盐胁迫下的表达分析[J].农业生物技术学报,2024,32(3):628-640,13.基金项目
国家现代贝类产业技术体系(CARS-49) （CARS-49）
宁波市重大科技攻关暨"揭榜挂帅"项目(2021Z114) （2021Z114）
