上海海洋大学学报2024，Vol.33Issue(1)：9-17,9.DOI:10.12024/jsou.20230304108
RseB影响溶藻弧菌感染花鲈的能力
RseB deletion affects the ability of Vibrio alginolyticus to infect Lateolabrax maculatus
摘要
Abstract
In order to study the effect of RseB gene deletion on the ability of Vibrio alginolyticus to infect the Lateolabrax maculatus,the LD50,colonization ability and immune response of the L.maculatus after infection were evaluated.The results showed that the LD50 of V.alginolyticus was 2.013×105 cfu/mL,and the LD50 of V.alginolyticus with RseB-deletion was 5.526×105 cfu/mL,and the virulence of RseB-deletion V.alginolyticus decreased by 63.6％.V.alginolyticus mainly colonized in the gill,intestine and skin of L.maculatus.Compared with V.alginolyticus,the degree of intestinal vacuolization and the damage of gill tissue were lower in L.maculatus after challenged with the RseB-deletion V.alginolyticus.Transcriptome sequencing was performed on the head kidney tissues of L.maculatus challenged by wild-type and RseB-deletion V.alginolyticus,respectively.A total of 236 immune-related differentially expressed genes were found,including 122 significantly up-regulated immune genes and 114 significantly down-regulated immune genes.Most of these genes were significantly enriched in specific immune pathways such as Th1 and Th2 cell differentiation,B cell receptor signaling pathway,T cell receptor signaling pathway,and some genes were significantly enriched in apoptosis-related pathways and inflammation-related NF-kappa B signaling pathway.In summary,the results show that RseB-deletion V.alginolyticus can cause immune response of host cells and reduce virulence.关键词
溶藻弧菌/花鲈/Rse基因/致病性/免疫响应Key words
Vibrio alginolyticus/Lateolabrax maculatus/RseB/pathogenicity/immune response分类
农业科技引用本文复制引用
周家宇,姜维,王雨果,陈小辉,罗土炎,鲍宝龙..RseB影响溶藻弧菌感染花鲈的能力[J].上海海洋大学学报,2024,33(1):9-17,9.基金项目
国家重点研发计划(2022YFD2400100) （2022YFD2400100）
福建省区域发展项目(2021N3016) （2021N3016）
福建省省属公益类科研院所基本科研专项(2020R1022009) （2020R1022009）
