上海海洋大学学报2024，Vol.33Issue(1)：230-241,12.DOI:10.12024/jsou.20221204042
杀鱼爱德华氏菌新型抗酸系统GadBCD的抗酸作用与致病作用
Acidity resistance and pathogenicity of GadBCD system in Edwardsiella piscicida
摘要
Abstract
In this study,we identified the important fish pathogenic bacterium,Edwardsiella piscicida,GadBCD,the strong acid resistance system and its function.Sequence analysis and co-transcription experiments showed that the GadBCD system consists of one glutamic acid decarboxylase,one glutaminase and three transporters,which form a manipulator.qRT-PCR revealed that gadBCD expression was largely unchanged upon strong acid and high temperature stimulation of bacteria,but significantly up-regulated upon hydrogen peroxide and host serum stimulation.A GadBCD system deletion mutant strain ΔgadP was constructed using homologous recombination,and experiments comparing the growth curve and survival rate under acidic stress of wild strain and ΔgadP revealed that the GadBCD system is not only an important player in the resistance of E.piscicida to medium to strong acids,but is also required for resistance to strong acids.GadBCD was found to be involved in bacterial virulence by comparing wild strain and ΔgadP in terms of biofilm formation,motility,resistance to host serum killing,and infection of cells.In conclusion,the GadBCD system is an important strong acid resistant system of E.piscicida and is involved in bacterial pathogenesis.关键词
杀鱼爱德华氏菌/谷氨酸脱羧酶系统/抗酸性/致病作用Key words
Edwardsiella piscicida/Glutamic acid decarboxylase system/acid resistance/pathogenicity分类
农业科技引用本文复制引用
高博,宫春光,舒艾梅,张杉杉,李静林,胡永华..杀鱼爱德华氏菌新型抗酸系统GadBCD的抗酸作用与致病作用[J].上海海洋大学学报,2024,33(1):230-241,12.基金项目
海南省自然科学基金(创新团队)项目(2019CXTD413) （创新团队）
