穴位不同层次和强度电刺激缓解肌肉炎性痛及对脊髓背角广动力神经元的抑制作用OA北大核心CSTPCD

Objective To observe the analgesic effects of different levels and intensities of electrical stimula-tion on the local acupoints in the pain source area and their impact on wide dynamic range(WDR)neurons in the spinal dorsal horn,in order to provide a basis for selecting appropriate parameters for electroacupuncture(EA)stimulation.Methods Wistar rats were used in 3 parts of the experiment.Complete Freund's adjuvant was used to establish a model of inflammation-induced pain in the gastrocnemius muscle.After modeling,6 rats were randomly selected for multi-channel extracellular electrophysiological recording of the electrical activity of WDR neurons,to determine the threshold for activating the A-component(Ta)and the C-component(Tc),which were used as the intervention intensities for skin transcutaneous electrical acupoint stimulation(TEAS)or EA.Thirty-six rats were randomly divided into normal,model,TEAS-Ta,TEAS-Tc,EA-Ta,and EA-Tc groups,with 6 rats in each group.In the pain source area,Ta or Tc intensity of TEAS or EA intervention at"Chengshan"(BL57)was performed for 30 min each time,once a day,for 3 consecutive days.A small animal pressure pain measurement instrument was used to measure the mechanical pressure pain threshold of the gastrocnemius muscle in rats,and the Von Frey filament was used to measure the mechanical pain threshold of the footpad.Thirteen rats were randomly selected to observe the im-mediate responsiveness of WDR neurons to Ta/Tc intensity of EA or TEAS in BL57.Results The thresholds of TEAS to activate WDR neuron A-component or C-component were(2.43±0.57)mA and(7.00±1.34)mA,respectively,while the thresholds for EA to activate muscle WDR neuron A-component or C-component were(0.72±0.34)mA and(1.58±0.35)mA,respectively.After injection of CFA into the gastrocnemius muscle,compared with the normal group both the mechanical pressure pain threshold of the gastrocnemius muscle and the mechanical pain threshold of the footpad of rats in the model group were significantly decreased(P<0.001).After TEAS-Ta,TEAS-Tc or EA-Ta intervention in the BL57,both the mechanical pressure pain threshold of the gastrocnemius muscle and the mechanical pain threshold of the footpad were significantly higher than those in the model group(P<0.05,P<0.001).Compared with the normal group,the electrical threshold for evoking WDR neuron C-component discharge was significantly decreased(P<0.001)in the model group,while increased after TEAS-Ta,TEAS-Tc,or EA-Ta intervention(P<0.01)compared with the model group.The evoked discharge frequency of muscle WDR neurons decreased significantly after immediate intervention with TEAS-Ta,TEAS-Tc,or EA-Ta(P<0.01,P<0.05).EA-Tc had no significant improvement on the evoked electrical activity of WDR neurons or pain behavior.Conclusion TEAS-Ta,TEAS-Tc,or EA-Ta can all alleviate the local and footpad mechanical pain in rats with muscle inflammation and inhibit the responsiveness of WDR neurons,indicating that different intensities are required for analgesic effects at different levels of acupoints in the pain source area.