针刺研究2024，Vol.49Issue(2)：119-126,8.DOI:10.13702/j.1000-0607.20230375
电针督脉对脊髓损伤大鼠脊髓组织线粒体融合及神经干细胞增殖分化的影响
Effect of electroacupuncture of Governor Vessel on mitochondrial fusion and proliferation and differentiation of neural stem cells in spinal cord injury rats
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)at"Dazhui"(GV14)and"Jizhong"(GV6)of the Governor Vessel(GV)on mitochondrial fusion and neural stem cell(NSC)proliferation and differentiation in the spinal cord of rats with spinal cord injury(SCI),so as to investigate its mechanisms underlying improvement of SCI.Methods SD rats were randomly divided into sham operation,model and EA groups,with 15 rats in each group.The SCI model was established by using a precision impactor.EA(20 Hz/100 Hz,1-2 mA)was applied to GV14 and GV6 for 30 min,once daily for 14 days.The rats'hindlimb locomotor function in each group was assessed using the Basso-Beattie-Bresnahan(BBB)locomotor scale.Histopathological changes of the injured spinal cord tissue and the number of neurons were evaluated after H.E.staining and Nissl staining.The expressions of Nestin,mitochondrial fusion-related protein optic atrophy-1(OPA1)and NSC markers sex-determining region Y-box 2(SOX2)in the injured spinal cord tissue were detected by immunofluorescence staining.The protein and mRNA expression levels of Nestin in the spinal cord tissue were detected by quantitative real-time PCR and Western blot,separately.Results Compared with the sham operation group,the BBB scores after modeling,and the number of neurons were significantly de-creased(P<0.001),while the mean fluorescence intensity values of Nestin,SOX2 and OPA1,and the expressions of Nestin mRNA and protein considerably increased(P<0.001,P<0.01,P<0.05)in the model group.After EA intervention and in comparison with the model group,the BBB scores at the 7th and 14th day,the number of neurons,the mean fluo-rescence intensity values of Nestin,SOX2 and OPA1,and the expressions of Nestin mRNA and protein were strikingly increased(P<0.05,P<0.01,P<0.001)in the EA group.H.E.staining showed swollen,ruptured and necrotic neurons of the spinal cord,with a large number of vacuoles and severe inflammatory cell infiltration after modeling,which was rela-tively milder in the EA group.Conclusion EA stimulation of GV14 and GV6 can promote the recovery of motor func-tion in rats with SCI,which may be related to its effects in promoting mitochondrial fusion and enhancing the prolifera-tion and differentiation of NSCs.关键词
电针/督脉/脊髓损伤/线粒体融合/神经干细胞/增殖分化Key words
Electroacupuncture/Governor Vessel/Spinal cord injury/Mitochondrial fusion/Neural stem cells/Proliferation and differentiation引用本文复制引用
吴明莉,段昭远,常文涛,高静,苏凯奇,冯晓东..电针督脉对脊髓损伤大鼠脊髓组织线粒体融合及神经干细胞增殖分化的影响[J].针刺研究,2024,49(2):119-126,8.基金项目
2021年河南省科技攻关项目(No.202102311130) （No.202102311130）
