电针对急性心肌梗死大鼠心肌电重构的影响及其机制研究OA北大核心CSTPCD
Effect of electroacupuncture on myocardial electrical remodeling in rats with acute myocardial infarction
目的:探讨电针"内关"通过增强瞬时外向钾电流(Ito)改善急性心肌梗死大鼠心肌电重构的作用机制.方法:雄性SD大鼠随机分成对照组、模型组和电针组,每组 10只.采用皮下注射异丙肾上腺素(85 mg/kg)诱导急性心肌梗死模型.电针组造模24 h后电针左侧"内关",每次20 min,每日1次,连续5 d.TTC染色观察心肌坏死情况;HE染色观察心肌组织病理形态、测量心肌细胞横截面积;心电图记录ST段高度、QT间期(QT)、校正QT间期(QTc),计算QT间期离散率(QTd)和校正QT间期离散率(QTcd);RNA测序法检测心肌组织钾离子通道相关基因表达差异;实时荧光定量PCR法和Western blot法分别检测心肌组织Kchip2、Kv4.2的mRNA和蛋白表达.结果:TTC染色可见对照组心肌呈红色,模型组大鼠心肌大部分呈白色缺血样改变,电针组仅见心尖处心肌组织呈白色.与对照组比较,模型组大鼠心肌细胞横截面积显著增大(P<0.01),ST段显著抬高(P<0.01),QT、QTc、QTd和QTcd均显著增加(P<0.05,P<0.01);与模型组比较,电针组大鼠心肌细胞横截面积显著减小(P<0.01),ST段显著下降(P<0.01),QT、QTc、QTcd和QTd均显著减少(P<0.01,P<0.05).RNA测序共筛选出20个3组共同表达的钾离子通道相关基因,电针组与模型组比较,其中Kchip2上调差异倍数最大.与对照组比较,模型组大鼠心肌组织Kchip2、Kv4.2 mRNA和蛋白表达均显著降低(P<0.01,P<0.05);与模型组比较,电针组大鼠心肌组织Kchip2、Kv4.2 mRNA和蛋白表达均显著升高(P<0.01,P<0.05).结论:电针可改善心肌梗死大鼠心肌电重构,其机制可能与增加Ito主要成分Kchip2、Kv4.2表达有关.
Objective To investigate the mechanism of electroacupuncture(EA)at"Neiguan"(PC6)in impro-ving myocardial electrical remodeling in rats with acute myocardial infarction(AMI)by enhancing transient outward po-tassium current.Methods A total of 30 male SD rats were randomly divided into control,model and EA groups,with 10 rats in each group.The AMI model was established by subcutaneous injection with isoprenaline(ISO,85 mg/kg).EA was applied to left PC6 for 20 min,once daily for 5 days.Electrocardiogram(ECG)was recorded after treatment.TTC staining was used to observe myocardial necrosis.HE staining was used to observe the pathological morphology of myocardial tissue and measure the cross-sectional area of myocardium.Potassium ion-related genes in myocardial tis-sue were detected by RNA sequencing.The mRNA and protein expressions of Kchip2 and Kv4.2 in myocardial tissue were detected by real-time fluorescence quantitative PCR and Western blot,respectively.Results Compared with the control group,cardiomyocyte cross-sectional area in the model group was significantly increased(P<0.01),the ST seg-ment was significantly elevated(P<0.01),and QT,QTc,QTd and QTcd were all significantly increased(P<0.05,P<0.01).After EA treatment,cardiomyocyte cross-sectional area was significantly decreased(P<0.01),the ST segment was significantly reduced(P<0.01),and the QT,QTc,QTcd and QTd were significantly decreased(P<0.01,P<0.05).RNA sequencing results showed that a total of 20 potassium ion-related genes co-expressed by the 3 groups were iden-tified.Among them,Kchip2 expression was up-regulated most notablely in the EA group.Compared with the control group,the mRNA and protein expressions of Kchip2 and Kv4.2 in the myocardial tissue of the model group were signifi-cantly decreased(P<0.01,P<0.05),while those were increased in the EA group(P<0.01,P<0.05).Conclusion EA may improve myocardial electrical remodeling in rats with myocardial infarction,which may be related to its functions in up-regulating the expressions of Kchip2 and Kv4.2.
萨喆燕;潘晓华;朱小香;兰彩莲;万隆;罗来;许金森
福建省中医药科学院经络研究所,福建省经络感传重点实验室,福州 350003
电针心肌梗死心肌电重构瞬时外向钾电流
ElectroacupunctureMyocardial infarctionMyocardial electrical remodelingTransient outward potassium current
《针刺研究》 2024 (002)
155-163 / 9
国家自然科学基金青年项目(No.81804001);福建省自然科学基金面上项目(No.2018J01858、2021J01912);福建省属公益类科研院所基本科研专项项目(No.2022R1003008)
评论