针刺研究2024，Vol.49Issue(2)：155-163,9.DOI:10.13702/j.1000-0607.20220908
电针对急性心肌梗死大鼠心肌电重构的影响及其机制研究
Effect of electroacupuncture on myocardial electrical remodeling in rats with acute myocardial infarction
摘要
Abstract
Objective To investigate the mechanism of electroacupuncture(EA)at"Neiguan"(PC6)in impro-ving myocardial electrical remodeling in rats with acute myocardial infarction(AMI)by enhancing transient outward po-tassium current.Methods A total of 30 male SD rats were randomly divided into control,model and EA groups,with 10 rats in each group.The AMI model was established by subcutaneous injection with isoprenaline(ISO,85 mg/kg).EA was applied to left PC6 for 20 min,once daily for 5 days.Electrocardiogram(ECG)was recorded after treatment.TTC staining was used to observe myocardial necrosis.HE staining was used to observe the pathological morphology of myocardial tissue and measure the cross-sectional area of myocardium.Potassium ion-related genes in myocardial tis-sue were detected by RNA sequencing.The mRNA and protein expressions of Kchip2 and Kv4.2 in myocardial tissue were detected by real-time fluorescence quantitative PCR and Western blot,respectively.Results Compared with the control group,cardiomyocyte cross-sectional area in the model group was significantly increased(P<0.01),the ST seg-ment was significantly elevated(P<0.01),and QT,QTc,QTd and QTcd were all significantly increased(P<0.05,P<0.01).After EA treatment,cardiomyocyte cross-sectional area was significantly decreased(P<0.01),the ST segment was significantly reduced(P<0.01),and the QT,QTc,QTcd and QTd were significantly decreased(P<0.01,P<0.05).RNA sequencing results showed that a total of 20 potassium ion-related genes co-expressed by the 3 groups were iden-tified.Among them,Kchip2 expression was up-regulated most notablely in the EA group.Compared with the control group,the mRNA and protein expressions of Kchip2 and Kv4.2 in the myocardial tissue of the model group were signifi-cantly decreased(P<0.01,P<0.05),while those were increased in the EA group(P<0.01,P<0.05).Conclusion EA may improve myocardial electrical remodeling in rats with myocardial infarction,which may be related to its functions in up-regulating the expressions of Kchip2 and Kv4.2.关键词
电针/心肌梗死/心肌电重构/瞬时外向钾电流Key words
Electroacupuncture/Myocardial infarction/Myocardial electrical remodeling/Transient outward potassium current引用本文复制引用
萨喆燕,潘晓华,朱小香,兰彩莲,万隆,罗来,许金森..电针对急性心肌梗死大鼠心肌电重构的影响及其机制研究[J].针刺研究,2024,49(2):155-163,9.基金项目
国家自然科学基金青年项目(No.81804001) （No.81804001）
福建省自然科学基金面上项目(No.2018J01858、2021J01912) （No.2018J01858、2021J01912）
福建省属公益类科研院所基本科研专项项目(No.2022R1003008) （No.2022R1003008）
