hsa-miR-204-5p对人脐静脉内皮细胞生物学行为的影响OA北大核心CSTPCD
Effects of hsa-miR-204-5p on biological behaviors of human umbilical vein endothelial cells
目的:探究hsa-miR-204-5p对人血管内皮细胞活力、迁移、周期及凋亡等生物学行为的影响.方法:使用人脐静脉内皮细胞株EA.hy926构建过表达hsa-miR-204-5p(hsa-miR-204-5p mimics)模型.通过细胞划痕、Transwell、CCK-8、细胞周期和凋亡率等指标评估hsa-miR-204-5p对内皮细胞功能状态的影响.接着,使用RNA测序和RT-qPCR寻找并验证hsa-miR-204-5p的下游靶基因,将RN…查看全部>>
AIM:This study aimed to investigate the effects of hsa-miR-204-5p on the viability,migration,cell cycle,and apoptosis of human vascular endothelial cells.METHODS:We established a model using the hsa-miR-204-5p mimic in the human umbilical vein endothelial cell line EA.hy926.We evaluated the effects of hsa-miR-204-5p on endothelial cell functionality through various analyses,including cell scratch,Transwell,CCK-8,cell cycle,and apopto-sis assays.Subsequently,…查看全部>>
张义炜;杨英;潘尚领
广西医科大学基础医学院病理生理学教研室,广西 南宁 530021广西医科大学基础医学院病理生理学教研室,广西 南宁 530021广西医科大学基础医学院病理生理学教研室,广西 南宁 530021
临床医学
hsa-miR-204-5p血管内皮细胞RNA测序MAPK信号通路
hsa-miR-204-5pvascular endothelial cellsRNA seqencingMAPK signaling pathway
《中国病理生理杂志》 2024 (2)
230-237,8
国家自然科学基金助项目(No.81960266)
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