中国农业大学学报2024，Vol.29Issue(3)：125-133,9.DOI:10.11841/j.issn.1007-4333.2024.03.11
鸡RUNX3基因组织表达及生物信息学分析
Tissue expression and bioinformatics analysis of the chicken RUNX3 gene
摘要
Abstract
To investigate the potential functions of the Runt-related transcription factor 3(RUNX3)gene,this study utilized quantitative real-time PCR(qRT-PCR)to detect the expression of the chicken RUNX3 gene in the heart,liver,spleen,leg and duodenum tissues.The evolutionary tree of the RUNX3 gene was constructed using bioinformatics software,and the physicochemical properties and structural functions of the encoded protein were analyzed.The results showed that:1)The expression level of the chicken RUNX3 gene was significantly higher in the spleen and duodenum compared to other tissues(P＜0.05).2)Phylogenetic analysis of the nucleotide sequences showed that chicken RUNX3 was most closely related to turkey and highly conserved.3)Physicochemical property analysis showed that chicken RUNX3 gene-encoded protein was a stable and hydrophilic protein.Structural domain prediction revealed that the protein did not have a transmembrane structure,contained two conserved structural domains,and the secondary and tertiary structure mainly consisted of random curls,and was predicted to contain 58 phosphorylation sites,31 serine sites,23 threonine sites,and 4 tyrosine sites.4)Protein interactions prediction revealed that RUNX3 was associated with SMAD3,CBFB,GATA3,EP300,SMAD2,CTNNB1 and other proteins.In conclusion,chicken RUNX3 was highly expressed in spleen and duodenum,its nucleotide sequence was highly conserved in avian species,RUNX3 protein was a stable,hydrophilic,non-transmembrane protein,and there was a strong interaction between RUNX3 and proteins such as SMAD3,CBFB,and GATA3,etc.The present study provides a theoretical basis for the further study of the mechanism of action of chicken RUNX3 at molecular level.关键词
鸡/RUNX3基因/组织表达谱/生物信息学分析Key words
chicken/RUNX3 gene/tissue expression profile/bioinformatics analysis分类
农业科技引用本文复制引用
杜文苹,曹然然,胡晓玉,张晨曦,许春红,杨岚,雷艳茹,康相涛,李文婷..鸡RUNX3基因组织表达及生物信息学分析[J].中国农业大学学报,2024,29(3):125-133,9.基金项目
河南省优秀青年科学基金项目(232300421034) （232300421034）
神农种业实验室一流课题(SN01-2022-05) （SN01-2022-05）
