中国农业大学学报2024，Vol.29Issue(3)：143-154,12.DOI:10.11841/j.issn.1007-4333.2024.03.13
西藏地区牦牛源沙门菌耐药表型和基因型相关性分析及分子特征分析
Correlation analysis and molecular characteristics analysis of drug resistance phenotype and genotype of Salmonella from yak in Tibet
摘要
Abstract
In order to analyze the drug resistance and molecular characteristics of Salmonella from yaks in Tibet,the drug resistance of 30 strains of Salmonella isolated from yak feces in Tibet were tested by drug-sensitive paper method with determination of resistance of 22 antibiotics including three major categories of lactams,quinolones and aminoglycosides.PCR amplification was used to detect 18 drug resistance genes such as bla-TEM,bla-CTX and bla-CMY.Chi-square test and Fisher Precise test were used to analyze the correlation between the expression forms of drug resistance and the carrying status of drug resistance genes.The related drug resistance genes were amplified and sequenced.CLC Sequence Viewer 7.5 and MAGE 7.0 software was used to analyze the relationship between the difference of key amino acid sites related to coding translation of β-lactam resistance gene(bla-TEM)and aminoglycoside resistance gene(armA)and drug resistance characteristics.The results showed that:1)The 30 isolates showed serious resistance to β-lactam,there was a relatively serious phenomenon of resistance to lactams,with 30 strains of Salmonella accounting for the most resistance to oxacillin(OX),accounted for 60％.They are also resistant to penicillin(PG),piperacillin(PRL),amoxicillin(AMX),amPicillin(AM),carben icillin(CB),cephalexin(CN),cefuroxime(CXM),ceftriaxone(CRO),cefadine(RAD),ceftazidime(CAZ),cefoperazone(CFP)and cefzolin(CZO).The resistance rate were 56.67％,43.33％,40.00％,53.33％,53.33％,46.67％,56.67％,53.67％,50.00％,46.67％56.67％and 30.00％,respectively.They were sensitive to quinolones.The resistance rate to strePtomycin(SM)in aminoglycoside accounted for 60％,while the resistance rates to kanamycin(KAN),tobramycin(CAS)and amikacin(AKM)were 13.33％,33.33％and 30.00％,respectively.2)The dominant drug resistance profile of 30 Salmonella strains was PG/OX/PRL/AMX/AM/CB/SM.3)The carrying rate of β-lactam related resistance gene bla-TEM was higher(53.3％,16/30).In addition,bla-TEM and β-lactam drugs showed positive effects on penicillin(PG),oxacillin(OX),piperacillin(PRL),amoxicillin(AMX),ampicillin(AM),Carbenicillin(CB),cefalexin(CN),cefuroxime(CXM),cefradine(RAD),ceftazidme(CAZ)and cefoperazone(CFP).There was a significant correlation(P＜0.05),the carrying rate of aminoglycosid-related resistance gene armA-was 60％(18/30),and it was significantly correlated with the resistance of tobramycin(CAS),streptomycin(SM)and Amikacin(AKM)(P＜0.05).4)Some amino acids encoded by bla-TEM and armA had differences sites related to the resistance of Salmonella bacteria.In summary,the situation of β-lactam drug and aminoglycoside drug resistance of Salmonella in Tibet is very serious.The reason for the high resistance rate of β-lactam drug and aminoglycoside drug in Tibet is that Salmonella bacteria carry resistance genes bla-TEM and armA,which are related to amino acid site mutations.This study provides a theoretical basis for strengthening the resistance monitoring of yak Salmonella in Tibet.关键词
沙门菌/β-内酰胺类/喹诺酮类/氨基糖苷类/分子特征Key words
Salmonella/beta-lactam/quinolones/aminoglycosides/molecular characterization分类
农业科技引用本文复制引用
李登宇,杨美琦,张开琴,李子轩,央拉,拜占春,索朗斯珠..西藏地区牦牛源沙门菌耐药表型和基因型相关性分析及分子特征分析[J].中国农业大学学报,2024,29(3):143-154,12.基金项目
财政部和农业农村部:国家现代农业产业技术体系项目(CARS-37) （CARS-37）
西藏自治区科技厅2019年度重点项目(XZ201902NB04) （XZ201902NB04）
2022年西藏自治区级大学生创新创业训练计划项目 （）
