中国农业大学学报2024，Vol.29Issue(3)：155-163,9.DOI:10.11841/j.issn.1007-4333.2024.03.14
乳酸杆菌对牦牛小肠黏膜屏障的保护作用及机制
Protective effect and mechanism of Lactobacillus on the mucosal barrier of the small intestine in yaks
摘要
Abstract
The aim of this study was to investigate the therapeutic effects and mechanisms of lactic acid bacteria(LAB)on intestinal mucosal barrier damage caused by E.coli in calf yaks.A total of 24 healthy calf yaks were randomly divided into four groups:control group,model group(E.coli O78),low-dose LAB group(E.coli O78+1×107 CFU LAB),and high-dose LAB group(E.coli O78+1×1011 CFU LAB).The morphologic evaluation of small intestine was processed by HE staining,and the ratio of villus height(VH)to crypt depth(CD)in each segment of small intestine was calculated(CN).The expression levels of p38 and MAPK proteins in small intestine were detected by Western blot.The results showed that:1)In the model group,calf yaks were treated with E.coli O78,the villi of small intestine of calf yaks were disorderly arranged,the crypt were deepen,and the boundary was fuzzy.There were inflammatory cell infiltration in the lamina propria and submucosa.After the intervention of LAB,the histopathological structure of small intestine villi of calf yaks were improved.2)The VH index and V/C ratio of small intestine in the model group were significantly lower than those in the control group.With the increase of LAB intervention dose,the VH index and V/C ratio of small intestine in the high-dose LAB group were significantly higher than those in the model group and the low-dose LAB group(P＜0.05),while the V/C ratio in the high-dose LAB group was significantly higher than that in the model group(P＜0.05),and there was no significant difference in VH index and V/C ratio between the high-dose LAB group and the control group(P＞0.05).3)Compared with the control group,the expression levels of p38 and MAPK proteins in the model group were significantly increased(P＜0.01),and the protein expression levels of p38 and MAPK decreased with the increase of LAB intervention dose.The expression levels of p38 and MAPK proteins in the high-dose LAB group were significantly lower than those in the model group and the low-dose LAB group,and there was no significant difference compared with the control group(P＞0.05).In conclusion,the LAB can improve the morphology and structure of small intestine,regulate the expression of p38 and MAPK proteins,and play a role in treatment of E.coli O78 induced diarrhea in calf yaks,which provides a theoretical basis and experimental basis for the research and development of probiotic products.关键词
牦牛/乳酸杆菌/大肠埃希氏菌/肠道黏膜/保护机制Key words
yak/Lactic acid bacteria/Escherichia coli/intestinal mucosa/protection mechanism引用本文复制引用
常振宇,董海龙,叶炳涛,陈永旭,曾江勇,吴庆侠..乳酸杆菌对牦牛小肠黏膜屏障的保护作用及机制[J].中国农业大学学报,2024,29(3):155-163,9.基金项目
国家自然科学基金地区科学基金项目(32160857) （32160857）
西藏自治区科技重大专项(XZ202101ZD0002N-05-01) （XZ202101ZD0002N-05-01）
