中国农业大学学报2024,Vol.29Issue(4):103-112,10.DOI:10.11841/j.issn.1007-4333.2024.04.09
甜玉米苗期耐铝性状QTL定位
QTL mapping of aluminum tolerance traits in sweet maize seedling stage
摘要
Abstract
In order to explore the aluminum tolerance gene resources in sweet maize,a hybrid combination'T49 × T56'was generated by taking two inbred lines'T49'(♀)and'T56'(♂),which have significant differences in aluminum tolerance characters,as the parents.A total of 208 F2 individual plants were used as genetic mapping population to construct the genetic linkage map of maize.Seedling height coefficient,seedling weight coefficient,root length coefficient,root weight coefficient and whole plant fresh weight coefficient were selected as the evaluation indexes.The aluminum tolerance of maize QTL related to aluminum resistance traits was detected by composite interval mapping.The results showed that:Ten SSR marker linkage groups were obtained on ten pairs of sweet maize chromosomes.The total length was 1 199.10 cM,and the average distance was 7.83 cM.A total of 3 QTLS for seedling height,4 QTLS for seedling weight,4 QTLS for root length,3 QTLS for root weight,and 3 QTLS for whole plant fresh weight were detected.One single QTL could explain 6.46% to 25.37% of phenotypic variation.In the labeled intervals phi065—umc1271(bin 9.03-9.04)on chromosome 9,a major QTL was detected in the coefficient of seedling height,seedling weight,root length,root weight and whole plant fresh weight,and the contribution rate was 13.1%-25.3%.Combined with GO and KEGG databases,two stable genes Zm00001d045472 and Zm00001d046391 related to abiotic stress tolerance of sweet corn were found in phi065-umc1271.关键词
甜玉米/耐铝/复合区间作图/QTLKey words
sweet corn/aluminum resistance/composite interval mapping/QTL分类
农业科技引用本文复制引用
孟起同,李明,周富亮,梁思维,刘家奇,宁丹,赵欣悦,刘鹏飞,蒋锋..甜玉米苗期耐铝性状QTL定位[J].中国农业大学学报,2024,29(4):103-112,10.基金项目
广东省农业农村厅农业科研类及技术推广示范类项目(粤财农[2023]189号) (粤财农[2023]189号)
2023年广东省普通高校自然科学类重点领域专项(2023ZDZX4017) (2023ZDZX4017)
广州市政农业农村项目(22304496) (22304496)
广东省阳江市2023年省科技创新专项资金(SDZX2023024) (SDZX2023024)