中国农业大学学报2024，Vol.29Issue(4)：205-214,10.DOI:10.11841/j.issn.1007-4333.2024.04.18
基于NIR定量分析模型快速预测酶解蒲公英中多糖的含量
Rapid prediction of polysaccharide content in enzymatically hydrolyzed dandelion based on NIR quantitative analysis model
摘要
Abstract
In order to quickly and real-time evaluate the quality of Chinese herbs in the production process of enzymatic hydrolysis,the study conducted enzymatic hydrolysis on 5 type of dandelion and a total of 125 samples of five kinds of dandelions were collected.Partial least squares regression(PLSR)method was used to establish the NIR analytical model for enzymatic hydrolysis of dandelion polysaccharides.The results showed as follows:1)When the spectrum was preprocessed by Standard Normal Variate(SNV)+Detrend+Second Derivative(SD),the spectrum range was 908-1 670 nm,and the principal factor number was 2,the modeling effect of enzymatic dandelion polysaccharide prediction model was the best.2)The correlation coefficient of the calibration set(R2c)was 0.917 5,the root mean square error of the calibration set(RMSEC)was 7.714 4,the standard error of the calibration(SEC)was 7.753 3,and the highest relative percent deviation of the calibration set(RPDc)was 3.480 9.The correlation coefficient of the validation set(R2p)was 0.878 4,the root mean square error of the cross validation set(RMSECV)was 9.411 5,the standard error of prediction(SEP)was 9.458 2,and the relative percent deviation of the validation(RPDp)was 2.867 2.3)The external test results showed that there was no significant difference between the predicted value and the measured value of the sample(P＞0.05).In summary,the NIR analytical model established in this study can be used for rapid and non-destructive real-time monitoring of the polysaccharide content of the enzymatic hydrolysis of dandelion,and can provide technical support for the quality control of the enzymatic hydrolysis process of dandelion.关键词
近红外光谱法/定量分析模型/多糖/酶解蒲公英Key words
near infrared spectroscopy/quantitative analysis model/polysaccharides/enzymatic hydrolysis of dandelion分类
农业科技引用本文复制引用
郑越,李霞,刘娜,王园,齐景伟,安晓萍..基于NIR定量分析模型快速预测酶解蒲公英中多糖的含量[J].中国农业大学学报,2024,29(4):205-214,10.基金项目
内蒙古自治区科技重大专项(2021ZD0023-3) （2021ZD0023-3）
内蒙古自治区科技计划项目(2022YFSJ0029) （2022YFSJ0029）
国家乳业技术创新中心项目(2022科研攻关-2) （2022科研攻关-2）
