中国农业大学学报2024，Vol.29Issue(4)：215-225,11.DOI:10.11841/j.issn.1007-4333.2024.04.19
贝莱斯芽孢杆菌和戊糖片球菌联合使用对饲喂霉变饲料肉鸡生产性能和肠道损伤的影响
Effect of combined use of Bacillus velezensis and Pediococcus pentosaceus on the production performance and intestinal damage of broilers fed with moldy feed
摘要
Abstract
To analyze the effects of the combination of Bacillus subtilis and Pediococcus pentosaceus tablets on the performance and intestinal damage of broilers fed moldy feed,96 1-day-old white feather broiler male chicks were randomly divided into a blank group,an attack group,a probiotic group and a detoxification group.Each group had 3 replicates,each with 8 chicks,and the test period was 21 d.The changes in body weight,feed intake and feed-to-weight ratio of broilers in different groups were counted and compared.Changes in serum biochemical indexes were detected by ELISA,and changes in the expression levels of proteins of the jejunum and inflammatory factor mRNA were detected by fluorescence PCR,and changes in the intestinal structure of broilers were observed by histopathology.The results showed that:1)Compared with the attack group,the body weight,average feed intake,and average daily weight gain of 21-day-old broilers in the detoxification group were significantly higher(P＜0.05).2)Compared with the attack group,the serum SOD activity,GSH-px activity and MDA content of broilers in the detoxification group were significantly decreased(P＜0.05),the T-AOC content was highly significantly increased(P＜0.01),and all the serum life indexes were significantly regressed(P＜0.05).3)Compared with the attack group,the morphology of jejunal villi of broilers in the detoxification group was significantly improved,the citrulline(CIT)content in jejunal tissues was highly significantly elevated(P＜0.01),and highly significant reductions in the contents of diamineoxidase(DAO),D-lactic acid and intestinal fatty acid binding protein(IFABP)occurred(P＜0.01).4)The mRNA expression levels of the jejunal tight junction protein-related genes occludin 1(ZO-1),occludin and claudin-1 were highly significantly increased in the detoxification group compared with the attack group(P＜0.01).5)Compared with the attack group,the mRNA expression levels of the broiler jejunal inflammatory factors IL-6,IL-8 and TNF-α were all highly significantly(P＜0.01)retuned in the detoxification group,and the mRNA expression level of IL-1β,was significantly(P＜0.05)retuned in the detoxification group.In conclusion,the combined use of Bacillus subtilis and Pediococcus pentosaceus can improve the decline in broiler performance caused by moldy feed,reduce the morphological changes,barrier damage and inflammatory response of the intestinal tract of broilers caused by moldy feed.This study can provide a theoretical and practical basis for the combined use of these two probiotics as feed additives in production practice to prevent and control mycotoxin poisoning.关键词
霉菌毒素/益生菌/肉鸡/空肠炎症/肠道屏障Key words
mycotoxins/probiotics/broilers/jejuno-intestinal inflammation/intestinal barrier分类
农业科技引用本文复制引用
李佳晖,王硕,龙淼..贝莱斯芽孢杆菌和戊糖片球菌联合使用对饲喂霉变饲料肉鸡生产性能和肠道损伤的影响[J].中国农业大学学报,2024,29(4):215-225,11.基金项目
国家自然科学基金项目(32273074 （）
31972746 （）
31872538 （）
31772809) （）
辽宁省教育厅重点项目(LJKZ0632) （LJKZ0632）
