植物学报2023，Vol.58Issue(6)：866-881,16.DOI:10.11983/CBB22261
玉米ZmICE2基因调控气孔发育
ZmICE2 Regulates Stomatal Development in Maize
摘要
Abstract
Plant epidermis is crucial in regulating photosynthesis,respiration,heat dissipation,and water utilization.Sig-nificant progress has been made in the study of stomatal development in dicotyledonous plants,such as Arabidopsis thaliana.Three important bHLH transcription factors(SPCH,MUTE,and FAMA)have been reported to be specifically expressed at different stages of cell division and differentiation in the stomatal lineage.They form heterodimers with an-other transcription factors SCRM/ICE1 and SCRM2/ICE2 to regulate the morphological transformation and changes of stomatal lineage cells across three stages of division,finally forming the stomatal complex.However,in monocots,espe-cially in Poaceae plants such as maize(Zea mays),studies on genes regulating epidermal morphogenesis are less re-ported.In this study,two single-gene recessive mutants,Zmice1-1(inducer of cbf expression1-1)and Zmice2-1,were isolated using reverse genetics approaches.Compared to the control B73,Zmice2-1 exhibited dwarfism,leaf chlorosis,reduced fertility,significantly lower stomatal density and index,disrupted arrangement of epidermal long cells,and ab-sence of spacing between stomata.Zmice1-1 leaves gradually turned yellow from the five-leaf stage and displayed com-plete chlorosis at later stages.The homozygous Zmice1-1 plants are growth-arrested and sterile,but the stomatal density showed no significant difference compared to the control.Different allels of Zmice2 were obtained using CRISPR-Cas9 genome editing technology.Phenotypic identification showed that Zmice2-2 had an abnormal stomatal phenotype similar to Zmice2-1,indicating that ZmICE2 is involved in the regulation of stomatal development.Transcriptome analysis of B73 and Zmice2-1 revealed that ZmICE2 primarily regulated stomatal development by affecting cell division and differentia-tion,participating in the formation of maize epidermal morphology.These results contribute to a better understanding of the mechanisms of epidermal morphogenesis in maize and provide valuable genetic resources for improving crop resil-ience and yield traits.关键词
玉米/气孔发育/气孔密度/表皮形态建成/CRISPR-Cas9基因编辑Key words
maize/stomatal development/stomatal density/epidermal morphogenesis/CRISPR-Cas9 gene editing引用本文复制引用
周文期,周玉乾,李永生,何海军,杨彦忠,王晓娟,连晓荣,刘忠祥,胡筑兵..玉米ZmICE2基因调控气孔发育[J].植物学报,2023,58(6):866-881,16.基金项目
河南大学省部共建作物逆境适应与改良国家重点实验室开放课题(No.2021KF04)、兰州大学细胞活动与逆境适应教育部重点实验室开放基金(No.lzujbky-2022-kb03)、国家自然科学基金(No.32160490,No.31860384)和甘肃省重大专项计划(No.21ZD11NA005,No.21ZD10NF003) （No.2021KF04）
