植物学报2023，Vol.58Issue(6)：882-892,11.DOI:10.11983/CBB23100
水稻茎秆细胞壁相关组分含量QTL定位及候选基因分析
Mapping of QTL for Cell Wall Related Components in Rice Stem and Analysis of Candidate Genes
摘要
Abstract
Lodging of rice is one of the main factors reducing rice yield.The mechanical strength of stem affects lodging resistance of rice and is closely related to the content of stem cell wall related components.Improving lodging resistance of rice by regulating the related components in the cell wall of stem is an effective way to improve the yield and quality of rice.In this study,indica rice variety Huazhan(Oryza sativa subsp.indica cv.'HZ')and ja-ponica rice variety Nekken2(O.sativa subsp.japonica cv.'Nekken2')were crossed to obtain F1 generation.A total of 120 RILs(recombinant inbred lines)population were obtained by successive multigeneration self-crossing and the genetic linkage map was constructed.Based on the constructed high-density genetic map,the QTLs related to the content of cellulose,hemicellulose,and lignin in the cell wall of rice stem were located and analyzed.The results showed that 4 QTLs related to cellulose,12 QTLs related to hemicellulose,and 8 QTLs related to lignin.At the same time,candidate genes analyses were conducted on the detected QTLs'intervals,and a total of 13 candidate genes were screened.The expression levels of candidate genes were detected by qRT-PCR.Except for LOC_Os02g58590 and LOC_Os12g41720,the other candidate genes showed significant differences between parents.The results laid an important foundation for exploring genes that regulate the mechanical strength of rice stems,further screening and breeding rice varieties with strong lodging resistance.关键词
水稻/茎秆机械强度/QTL定位/候选基因/抗倒伏Key words
Oryza sativa/mechanical strength of stem/QTL mapping/candidate genes/lodging-resistant引用本文复制引用
贾绮玮,王跃星,饶玉春,钟芊芊,顾育嘉,陆天麒,李玮,杨帅,朱超宇,胡程翔,李三峰..水稻茎秆细胞壁相关组分含量QTL定位及候选基因分析[J].植物学报,2023,58(6):882-892,11.基金项目
浙江省自然科学基金重点项目(No.LZ23C130003)和浙江省大学生科技创新活动计划暨新苗人才计划(No.2023R404040,No.2023R404041) （No.LZ23C130003）
