植物学报2023，Vol.58Issue(6)：893-904,12.DOI:10.11983/CBB22231
万寿菊舌状花花冠裂片突变体的形态鉴定及连锁标记开发
Morphological Identification and Development of Linkage Markers for Lobed Ray Floret Mutants in Marigold(Tagetes erecta)
摘要
Abstract
Marigold(Tagetes erecta)is an important ornamental plant of the genus Tagetes in the Asteraceae.The morphology of its ray floret corolla varies from smooth,undulate to different degree of incision.An F2 population was constructed from a cross between the mutant marigold JH with five-lobed corollas and fertile stamens in the first round florets and the inbred line S5 with smooth corolla of ray florets and aborting stamens.Inheritance analysis showed that the mutation trait of JH was controlled by a single-dominant gene,named Tagetes erecta lobed ray florets(Telf).Histological and cytological observations showed that the first round ray florets of JH were disk-like,with five lobes formed at the top of the corolla tube,the middle and lower parts were tubular,and the development of anthers and pollen grains are to normal.Using BSR-seq and comparative genomics methods,the gene Telf controlling lobed ray florets was mapped in the interval of 37003-SCAR and 34032-CAPS.37003-SCAR marker was 3.684 cM from the Telf gene.On the other side of the gene,34032-CAPS marker was placed about 3.517 cM from Telf.This study laid a foundation for the subsequent fine mapping of the target gene Telf,and also provided a new method for the selection of molecular markers in marigold.关键词
万寿菊/舌状花/裂片/BSR-seq/分子标记Key words
Tagetes erecta/ray floret/lobed corolla/BSR-seq/molecular marker引用本文复制引用
王文静,何燕红,朱钰,张洪铭,韦陆丹,易庆平,余晓敏,刘雨菡,张莉雪,程文翰..万寿菊舌状花花冠裂片突变体的形态鉴定及连锁标记开发[J].植物学报,2023,58(6):893-904,12.基金项目
国家自然科学基金(No.32172616)和特色花卉生物育种湖北省工程研究中心开放课题(No.2022ZD007) （No.32172616）
