植物学报2023，Vol.58Issue(6)：917-925,9.DOI:10.11983/CBB22228
富含紫杉烷类红豆杉的离体培养
In vitro Culture of Taxus Rich in Taxanes
摘要
Abstract
Taxus contains the anti-cancer ingredient paclitaxel,is the top-protected endangered plant in China,and has great medicinal value but scarce resources.To solve the problem of paclitaxel source shortage,in this study,an in vitro culture of a taxane-rich Taxus system was established.The results showed that MS+2 mg·L-1 6-BA+0.4 mg·L-1 NAA+0.7 g L-1 Pro was the optimal treatment for absorption surface expansion,and the expansion rate reached 90％.The adven-titious bud high-frequency synchronous growth induction was in the DCR+1 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 Glu+1 g·L-1 AC.The best biomass amplification phase Ⅰ was in MS+0.7 mg·L-1 6-BA+0.07 mg·L-1 NAA+0.1 mg·L-1 Phe+50 mg·L-1 PG,in which the biomass reached 542 mg.The optimized biomass amplification phase Ⅱ was obtained in MS+0.5 mg·L-1 6-BA+0.05 mg·L-1 NAA+0.1 mg·L-1 Phe+2 g.L-1 AC,in which the biomass reached 1 612 mg.The content of taxanes in tissue culture materials is much higher than that of natural materials.The content of paclitaxel in tissue cul-ture materials was 6.1 times that of natural materials.The contoent of baccatin Ⅲ in tissue culture materials was 8.2 times that of natural materials.The content of 10-DAB in tissue culture materials was 68.1 times that of natural materials.This study established an in vitro culture system of taxane-rich Taxus,and solved the problem of paclitaxel drug shortage.关键词
红豆杉/吸收面/高频同步/离体富集/紫杉烷类Key words
Taxus spp./absorbing surface/high-frequency synchronous growth/in vitro enrichment/taxanes引用本文复制引用
冯晓晖,闫学彤,郑珂媛,周强,张伟中,王权勇,朱木兰..富含紫杉烷类红豆杉的离体培养[J].植物学报,2023,58(6):917-925,9.基金项目
上海市绿化和市容管理局科研专项(No.G212403) （No.G212403）
