植物学报2023，Vol.58Issue(6)：926-934,9.DOI:10.11983/CBB23060
手指柠檬茎段离体再生体系建立
Establishment of In Vitro Regeneration System of Citrus australasica
摘要
Abstract
In order to establish the regeneration system of Citrus australasica,the effects of different plant growth regu-lators combinations,medium types and dark culture time on callus induction and plant regeneration of C.australasica were studied using stem segments as explants.The results showed that the best medium for adventitious bud induction was 1/2MS+4.0 mg·L-1 ZT+30.0 g·L-1 sucrose.Dark culture for 14 days and then light culture had the best promoting effect.The induction rate of callus and adventitious bud was 100％,and the average number of adventitious bud rege-neration per explants was 4.83.The optimal rooting medium was 1/2MS+0.5 mg·L-1 NAA,and the regenerated plants with the 94.43％rooting rate were obtained,and the average number of roots was 3.9.In the mixture of grass carbon∶perlite∶vermiculite=2∶1∶1(v/v/v),tissue culture seedlings had the best growth,and the survival rate was more than 90％.This study established in vitro regeneration system of C.australasica,which laid the foundation for the genetic improvement and rapid propagation of C.australasica fine varieties.关键词
手指柠檬/茎段/组织培养/离体再生Key words
Citrus australasica/stem segment/tissue culture/in vitro regeneration引用本文复制引用
谢纯刚,刘哲,章书声,胡海涛..手指柠檬茎段离体再生体系建立[J].植物学报,2023,58(6):926-934,9.基金项目
浙江省自然科学基金(No.LY19C130003) （No.LY19C130003）
