中药材2023，Vol.46Issue(5)：1118-1124,7.DOI:10.13863/j.issn1001-4454.2023.05.009
江南卷柏与混淆品旱生卷柏和兖州卷柏的鉴别研究
Identification of Selaginella moellendorffii and Adulterants Selaginella stauntoniana,Selaginella involvens
摘要
Abstract
Objective:To study the difference of morphological characteristics and HPLC typical chromatogram of Selaginella moel-lendorffii and adulterants Selaginella stauntoniana,Selaginella involvens,so as to find a solution for identification of Selaginella moellen-dorffii and its related species.Methods:Morphological characteristics of Selaginella moellendorffii and adulterants were abserved and compared.HPLC method was used,similarity software was used to calculate the similarity of its HPLC chromatogram,fingerprint regions with identification significance were selected,amentoflavone chromatographic peak was taken as reference,relative retention time of chromatographic peak was calculated,and the characteristic chromatogram was established.Chemical components of the characteristic chromatogram of Selaginella moellendorffii were identified by HPLC-DAD-ESI-MS/MS.And the chromatogram of its related species was compared.Results:Selaginella moellendorffii,Selaginella stauntoniana and Selaginella involvens had their own characteristics.HPLC chromatogram method was well investigated.The similarity of 10 batches of Selaginella moellendorffii samples ranged from 0.871 to 0.985,with stable common peaks.The chemical constituents of 13 kinds of flavonoids of Selaginella moellendorffii were identified.The characteristic chromatogram of Selaginella moellendorffii was obviously different from that of Selaginella stauntoniana and Selaginella in-volvens,which had the closest taxonomic relationship.Robusta diflavone and 4'-methoxy robusta-flavone were first discovered from Sela-ginella stauntoniana.Conclusion:This method is simple,reliable and practical,and can accurately identify Selaginella moellendorffii and its related adulterants,which provide a scientific basis for identification of Selaginella moellendorffii.关键词
江南卷柏/旱生卷柏/兖州卷柏/鉴定/黄酮类/HPLCKey words
Selaginella moellendorffii Hieron./Selaginella stauntoniana Spring/Selaginella involvens(Sw.)Spring/Identifica-tion/Flavonoid/HPLC分类
医药卫生引用本文复制引用
朱田密,陈科力,陈树和..江南卷柏与混淆品旱生卷柏和兖州卷柏的鉴别研究[J].中药材,2023,46(5):1118-1124,7.基金项目
第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
