中药材2023，Vol.46Issue(5)：1261-1266,6.DOI:10.13863/j.issn1001-4454.2023.05.033
甘草水提物促进Erastin诱导CRC细胞株发生铁死亡
Study on Erastin Induced Iron Death in CRC Cell Lines by Glycyrrhizae Radix et Rhizoma Water Extract
摘要
Abstract
Objective:To investigate whether Glycyrrhizae Radix et Rhizoma water extracts could promote iron death in Erastin treated colorectal cancer(CRC)cells.Methods:Two kinds of CRC cells(Caco-2 and SW837)were randomly divided into 5 groups,in-cluding normal control group,Glycyrrhizae Radix et Rhizoma water extract low(50 µg/mL),high(100 μg/mL)concentration groups,Glycyrrhizae Radix et Rhizoma water extract high concentration(100 µg/mL)with iron death attractor Erastin(30 µmol/L)group,Gly-cyrrhizae Radix et Rhizoma water extract high concentration(100 μg/mL)with Erastin(30 μmol/L)and Fer-1(30 nmol/L)group.Cell viability was determined by MTT,the cell apoptosis,death,and ROS production,mitochondrial membrane potential changes were determined by flow cytometry,cell death was verified by CCK-8 assay,Fe2+,MDA,GSH levels and SLC7A11,DPP4,NOX1,GPX4 pro-tein expressions were measured.Results:Compared with normal control group,programmed necrotizing inhibitor of apoptosis Necrostatin-1,apoptosis inhibitor Z-VAD-FMK and autophagy inhibitor 3-MA could not block cell death induced by Glycyrrhizae Radix et Rhizoma water extract combined with Erastin treatment.Ferroptosis inhibitor Fer-1 inhibited cell death caused by Glycyrrhizae Radix et Rhizoma water extract combined with Erastin,indicating that cell death was iron death.ROS production,MDA content,Fe2+content and DPP4 protein expression in Caco-2 and SW837 cells of Glycyrrhizae Radix et Rhizoma water extract(100 μg/mL)group and Glycyrrhizae Radix et Rhizoma water extract(100 µg/mL)with Erastin group were significantly increased.Mitochondrial membrane potential,GSH level and SLC7A11,NOX1,GPX4 protein expressions were significantly decreased(P＜0.05 or P＜0.01).Compared with Glycyrrhizae Radix et Rhizoma water extract(100 μg/mL)with Erastin group,the addition of Fer-1 significantly decreased ROS production,MDA content,Fe2+content and DPP4 protein expression in Caco-2 and SW837 cells.Mitochondrial membrane potential,GSH level and SLC7A11,NOX1,GPX4 protein expressions were significantly increased(P＜0.05 or P＜0.01).Conclusion:Glycyrrhizae Radix et Rhi-zoma water extract can induced iron death in CRC cell lines.关键词
甘草水提物/Erastin/结直肠癌/铁死亡Key words
Glycyrrhizae Radix et Rhizoma water extracts/Erastin/Colorectal cancer(CRC)/Iron death分类
医药卫生引用本文复制引用
尚韬,王城磊,庞石凯..甘草水提物促进Erastin诱导CRC细胞株发生铁死亡[J].中药材,2023,46(5):1261-1266,6.基金项目
浙江省中医药科技计划项目(2021ZB095) （2021ZB095）
