海南医学院学报2024，Vol.30Issue(4)：241-247,7.DOI:10.13210/j.cnki.jhmu.20231211.001
基于SOX9/NF-κB/MMP-13信号通路探讨补肾痹通方含药血清对IL-1β诱导软骨细胞凋亡的影响
Exploring the effect of Bushen Bitong recipe-containing serum on IL-1β-induced chondrocyte apoptosis based on SOX9/NF-κB/MMP-13 signaling pathway
摘要
Abstract
Objective:To observe the effect and possible mechanism of action of Bushen Bitong recipe(BSBT)containing serum on IL-1β-induced chondrocyte apoptosis.Methods:Chondrocytes of the 3rd generation rat were randomized into Control,IL-1β,IL-1β+BSBT(L),IL-1β+BSBT(M),and IL-1β+BSBT(H)groups(5%,10%and 15%BSBT-containing se-rum).The cell proliferation and apoptosis rate were tested 24 h after intervention;Western blot was used to detected the expres-sion levels of Bcl-2,BAX,Caspase-3,SOX9,NF-κB p65 and MMP-13 proteins in chondrocytes;ELISA was used to detected the levels of TNF-α,IL-6,and bFGF in the supernatants of chondrocyte culture.Results:Compared with Control group,cell proliferation activity decreased,apoptosis rate increased,NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level increased,and SOX9 protein level and bFGF level decreased in IL-1β group;compared with IL-1β group,different concentrations of BSBT-containing serum group,cell proliferation activity increased,and apoptosis rate decreased.NF-κB p65,MMP-13 protein level and TNF-α,IL-6 level decreased,SOX9 protein level and bFGF level increased;compared with IL-1β+BSBT(L)group,cell pro-liferation activity increased,apoptosis rate decreased in IL-1β+BSBT(M)and IL-1β+BSBT(H)groups,and NF-κB p65,MMP-13 protein level and TNF-α level and IL-6 levels decreased,and SOX9 protein levels and bFGF levels increased.Conclu-sion:Bushen Bitong recipe-containing serum may promote IL-1β-induced proliferation of chondrocytes,reduce apoptosis,im-prove the microenvironment of chondrocytes,and promote cartilage repair through the SOX9/NF-κB/MMP-13 signaling pathway.关键词
补肾痹通方/骨关节炎/软骨细胞/信号通路/IL-1βKey words
Bushen Bitong recipe/Osteoarthritis/Chondrocytes/Signaling pathway/IL-1β分类
医药卫生引用本文复制引用
易林,张文豪,向文远,石正誉,热米拉·艾买提,邓迎杰,方锐..基于SOX9/NF-κB/MMP-13信号通路探讨补肾痹通方含药血清对IL-1β诱导软骨细胞凋亡的影响[J].海南医学院学报,2024,30(4):241-247,7.基金项目
This study was supported by National Natural Science Foundation of China(82360934) （82360934）
Science and Technology Innovation Leading Talents Project of Xinjiang Uygur Autonomous Region(2022TSYCLJ0007) （2022TSYCLJ0007）
Xinjiang Uygur Autonomous Region Key Research and Development Task Special Project(2021B03006) （2021B03006）
Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01C170 （）
2022D01C171)国家自然科学基金(82360934) （82360934）
新疆维吾尔自治区科技创新领军人才项目(2022TSYCLJ0007) （2022TSYCLJ0007）
新疆维吾尔自治区重点研发任务专项(2021B03006) （2021B03006）
新疆维吾尔自治区自然科学基金(2022D01C170 （）
2022D01C171) （）
