前列腺素D2调控自噬影响胃癌干细胞的干性OACSTPCD
Prostaglandin D2 regulation of autophagy affects stemness of gastric cancer stem cells
目的:探究前列腺素D2(Prostaglandins D2,PGD2)对胃癌干细胞(gastric cancer stem cells,GCSCs)干性的影响及机制.方法:通过无血清培养法富集7901-GCSCs;然后通过流式细胞术检测无血清培养的7901-GCSCs干性标志物CD44的阳性率;通过干细胞成球实验检测不同浓度PGD2(2.5、5、10)μg/mL刺激后的成球能力,并采用Western blot实验检测不同浓度PGD2刺激后干性相关…查看全部>>
Objective:To explore the effect and mechanism of prostaglandins D2(PGD2)on the stemness of gastric can-cer stem cells(GCSCs).Methods:7901-GCSCs were enriched by serum-free culture method;then the positivity rate of CD44,a stemness marker,was detected by flow cytometry in serum-free cultured 7901-GCSCs;the sphere-forming ability was detected by the sphere-forming assay after stimulation with different concentrations of PGD2(2.5,5,10)μg/mL,and the expression o…查看全部>>
田恒金;汪非凡;高培垚;陈阿敏;王娜;张强
蚌埠医科大学第一附属医院检验科,安徽 蚌埠 233004||蚌埠医科大学肿瘤基础研究与临床检验诊断重点实验室,安徽 蚌埠 233004海军军医大学第一附属医院输血科,安徽 蚌埠 233004蚌埠医科大学肿瘤基础研究与临床检验诊断重点实验室,安徽 蚌埠 233004蚌埠医科大学第一附属医院检验科,安徽 蚌埠 233004蚌埠医科大学第一附属医院检验科,安徽 蚌埠 233004蚌埠医科大学第一附属医院检验科,安徽 蚌埠 233004
临床医学
PGD2胃癌胃癌干细胞自噬干性
PGD2Gastric cancerGastric cancer stem cellsAutophagyStemness
《海南医学院学报》 2024 (4)
255-261,7
This study was supported by the Natural Science Foundation of Anhui Province(1908085MH258)Scientific Research and Innovation Project of Bengbu Medical College(Byycxz21004)安徽省自然科学基金面上项目(1908085MH258)蚌埠医学院科研创新项目(Byycxz21004)
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