海南医学院学报2024，Vol.30Issue(4)：262-272,11.DOI:10.13210/j.cnki.jhmu.20231214.002
膀胱癌铜死亡相关免疫检查点基因预后特征和免疫与药物治疗分析
Prognostic characterization of copper death-related immune checkpoint genes and analysis of immunologic and pharmacologic therapy in bladder cancer
摘要
Abstract
Objective:Copper death-induced tumor cell death and immune checkpoint blockade therapy are highly selec-tive.Combining their advantages and understanding their characteristics in bladder cancer is very important for the development of new targeted therapy.The identification of bladder cancer by screening the characteristic genes of copper death-related immune checkpoints provides a theoretical basis for the selection of adjuvant treatment options and the application of new targets.Methods:The expression samples of normal bladder tissue and bladder cancer were obtained from TCGA and GEO databases,and 13 cop-per death genes and 79 immune checkpoint genes were extracted from previous studies.The mRNA expression of prognostic genes was verified by qPCR.The copper death-related immune checkpoint genes were screened by correlation analysis to construct a prognostic model,and the differences in the efficacy of immunotherapy and chemotherapy between the high-risk group and the low-risk group were evaluated.Results:A prognostic model consisting of BTNL9,CD160,TNFRSF14 and TNFRSF18 was constructed.Its reliable predictive ability was proved in both databases,and qPCR showed that the expression levels of the four genes were significantly different between the normal group and the cancer cell group.The effect of immunotherapy in the low-risk group was better than that in the high-risk group.Patients in the high-risk group had better chemotherapy efficacy.Conclusion:The copper death-related immune checkpoint gene model can accurately predict the prognosis of patients.Drug and immune analy-sis provide a basis for clinical treatment,and the discovery of potential targets provides a new solution for clinical decision-making.关键词
膀胱癌/铜死亡/免疫检查点/免疫治疗/药物治疗Key words
Bladder cancer/Copper death/Immune checkpoints/Immunotherapy/Drug therapy分类
医药卫生引用本文复制引用
杨丛语,阿茹娜,刘嘉铭..膀胱癌铜死亡相关免疫检查点基因预后特征和免疫与药物治疗分析[J].海南医学院学报,2024,30(4):262-272,11.基金项目
This study was supported by Key Research Project of Sichuan Provincial Department of Science and Technology(23ZDYF1246)四川省科技厅重点研究项目(23ZDYF1246) （23ZDYF1246）
