海南医学院学报2024，Vol.30Issue(4)：287-296,10.DOI:10.13210/j.cnki.jhmu.20231009.002
基于网络药理学和药效学探讨扶阳解表颗粒抗病毒性肺炎的作用机制
To explore the mechanism of Fuyang Jiebiao granules against viral pneumonia based on network pharmacology and pharmacodynamics
摘要
Abstract
Objective:To investigate the mechanism of Fuyang Jiebiao granule(FYJBKL)in the treatment of viral pneu-monia.Methods:Firstly,a network model was constructed using network pharmacology to study the target expression sites of FYJBKL viral pneumonia,so as to determine the main targets and important signal transduction pathways for the treatment of vi-ral pneumonia.Secondly,the main components of the drug and the main target are docked.Then,the fever,sweating and inflam-mation rat models were established to explore the antipyretic,sweating and anti-inflammatory mechanisms of FYJBKL.Finally,the contents of IL-17,IL-1 β,TNF-α and IL-6 in blood samples of rats were analyzed by ELISA method,and the morphological changes of lung tissue were observed by HE staining.Results:Quercetin,luteolin,kaempferol,etc,and the main mechanism tar-gets are IL-17,IL-1 β,TNF-α,IL-6 and so on.Thirty signal pathways were identified by KEGG enrichment analysis,including IL-17 signaling pathway,human cytomegalovirus infection pathway,Kaposi's sarcoma associated herpesvirus infection pathway and so on.After the study of molecular docking,we found that the contact efficiency between active substances and possible key targets was good.The high and middle concentration groups of FYJBKL significantly decreased the expression of IL-17,IL-1 β,TNF-α and IL-6 in the blood of rats with inflammation(P<0.05).FYJBKL significantly reduced the foot swelling induced by egg white and inhibited the increase of body temperature induced by yeast in rats(P<0.05).HE staining showed that FYJBKL im-proved fibrosis and inflammatory exudation in lung tissue to varying degrees.Conclusion:The effects of FuyangJiebiao granules on the related signal pathways of anti-virus,anti-immune and anti-inflammation as well as biological and cellular processes may be caused by the binding of quercetin,luteolin,kaempferol and other active ingredients to their shared targets.Fuyang Jiebiao gran-ules can improve the related symptoms caused by viral pneumonia,and its mechanism may be related to the activities of TNF,IL-17,IL-6 and other related channels,which are multiple targets of inflammation regulation.关键词
扶阳解表颗粒/网络药理学/药效学/病毒性肺炎/分子对接/经方扶阳Key words
FYJBKL granule/Network pharmacology/Pharmacodynamics/Viral pneumonia/Molecular docking/Jing-fang Fuyang分类
医药卫生引用本文复制引用
谭丹丹,冯振宇,孟霜,王旭艳,王鑫鑫,赵杰,赵建平..基于网络药理学和药效学探讨扶阳解表颗粒抗病毒性肺炎的作用机制[J].海南医学院学报,2024,30(4):287-296,10.基金项目
This study was supported by Emergency Research Project for Novel Coronavirus(2019-nCoV)Prevention and Control in Shanxi Province(202003D31012/GZ) （2019-nCoV）
Jingfang Fuyang Key Laboratory of Shanxi Province(202104010910011) （202104010910011）
Shanxi Provincial Health Commission Key Laboratory Construction Project山西省新型冠状病毒(2019-nCoV)疫情防控紧急科研攻关项目(202003D31012/GZ) （2019-nCoV）
经方扶阳山西省重点实验室(202104010910011) （202104010910011）
山西省卫生健康委员会重点实验室建设计划项目 （）
