华北农学报2024，Vol.39Issue(1)：175-183,9.DOI:10.7668/hbnxb.20194347
抗(感)赤霉病谷子品种内生菌多样性分析
Analysis of Endophytic Bacteria Diversity in Resistant(Susceptible)Foxtail Millet Varieties to Fusarium Head Blight
摘要
Abstract
To reveal the diversity characteristics of endophytic bacteria in different cultivars and organs,and clarify the correlation between endophytic bacteria community structure and host varieties,organ types and disease resistance and susceptible characteristics.Six different varieties of foxtail millet,Jigu 22,Honggu,Longgu No.11 and Xiaoqinggu,Shiliuzi and Nenxuan 16,which were resistant(susceptible)to Fusarium head blight,were selected as materials.The leaves,roots,stems,leaf sheaths and mature spikes of foxtail millet,were taken respectively.DNA was extracted for PCR amplification of 16S rDNA V3—V4 region,and Miseq library was constructed for high-throughput sequencing.Microbial diversity was analyzed by Major biological cloud platform.There were certain differences in endophyte species composition between susceptible and resistant varieties,the dominant populations of endophytes were Proteobacteria and Actinobacteriota,followed by Bacteroidota and Firmicutes,Myxococota and Chloroflexi were lower relative abundance followed by Gemmatimondota and Fusobacteriota.Alpha diversity analysis showed that the resistant cultivars had higher panicle community richness at leaf and maturity spikes and higher panicle diversity at leaf,root and maturity spikes.The susceptible varieties had higher richness of root and stem community and higher diversity of stem and leaf sheath;PCoA analysis showed that organ type had more effect on endophytic community structure than variety.Species composition analysis showed that the diversity of endophytic flora in foxtail millet was affected by different varieties and different parts,and the species of endophytic flora in different parts were quite dif-ferent.The diversity of endophytic flora between susceptible cultivars Xiaoqinggu,Shiliuzi and Nenxuan 16 and re-sistant cultivars Jigu 22,Honggu and Longgu No.11 was quite different.The resistant cultivars had NB1-j in leaf sheath stage,and the ears had Acidobacteriota in mature stage.The study showed that the diversity and community structure of endophytic bacteria were affected by different organs and varieties of the foxtail millet,and the organ types had more influence on the community structure of endophytic bacteria than varieties.关键词
谷子/谷子赤霉病/内生菌/多样性Key words
Foxtail millet/Fusarium head blight/Endogenic bacteria/Diversity分类
农业科技引用本文复制引用
毛亚楠,田菲菲,刘婷婷,韩欣芃,郝志敏,李志勇..抗(感)赤霉病谷子品种内生菌多样性分析[J].华北农学报,2024,39(1):175-183,9.基金项目
河北省农林科学院基本科研业务包干制项目(HBNKY-BGZ-02) （HBNKY-BGZ-02）
河北省自然科学基金项目(C2021301062) （C2021301062）
河北省农林科学院基本业务费(2021030201) （2021030201）
