海马过表达Ephrin-B3对颞叶癫痫大鼠突触重塑的影响OACSTPCD

Objective To investigate the possible mechanism of overexpression of tyrosine protein kinase B3(Ephrin-B3)in the hippocampus on synaptic remodeling in rats with epilepsy.Methods Forty male SD rats were randomly divided into blank control group,epilepsy group,empty carrier group and lentivirus Efnb3 overexpression group,with 10 rats in each group.Epilepsy modeling was performed in all the other three groups except the blank group.One week before modeling,LV5-NC(5 μL)vector was injected into the hippocampus on both sides of the empty vector group,and Efnb3 lentivirus(5 μL)was injected into the hippocampus of the lentivirus overexpression group.Ephrin-B3,postsynaptic densitin-95(PSD95)and ion glutamate receptor subunit(NR2B)were detected by qPCR from hippocampal tissues taken from each group 24 hours after the seizure model,while the expressions of PSD95 and NR2B were detected by western blotting.Results The seizure latency of Ephrin-B3 overexpression group(30.2±4.38)minutes was significantly longer than that of Ephrin-B3 overexpression group(22.4±3.91)minutes and empty vector group(21.0±5.29)minutes(P<0.05).The mRNA tables of Ephrin-B3,PSD95 and NR2B in the epileptic and empty vector groups after the model were constructed.The mRNA relative expressions of Ephrin-B3 transfected group were significantly increased compared with those of the epilepsy model group(Ephrin-B3:F=25.11,P=0.002 7;PSD95:F=14.80,P=0.020 3;NR2B:F=19.51,P=0.001 0)and empty vector group(Ephrin-B3:P=0.002 9;PSD95:P=0.0160;NR2B:P=0.0034).The relative expressions of histone in Ephrin-B3 transfected group increased compared with those in the epileptic model(Ephrin-B3:F=17.72,P=0.003 2;PSD95:F=7.889,P=0.014 5;NR2B:F=9.755,P=0.019 9)and empty vector group(Ephrin-B3:P= 0.0034;PSD95:P=0.025 3;NR2B:P=0.014 4).Conclusion Overexpression of Ephrin-B3 can alleviate the injury of epileptic seizure,which may be related to regulating the expression of postsynaptic proteins PSD95 and NR2B to control synaptic remodeling.