海洋科学2023，Vol.47Issue(11)：66-78,13.DOI:10.11759/hykx20230720001
海南万泉河口尖鳍鲤线粒体基因组测序与结构特征分析
Mitochondrial genome sequencing and structural characteristic analysis of Cyprinus acutidorsalis from Wanquan Estuary,Hainan
摘要
Abstract
In this study,the complete mitochondrial genome sequence of Cyprinus acutidorsalis from the Wanquan River Estuary of Hainan Province is obtained using second-generation sequencing.Their structural characteristics are also analyzed.The results show a typical circular closed-loop double-stranded molecule with a total length of 16 581 bp.The base composition is A(31.96％),G(15.69％),C(27.53％),and T(24.82％),which contains 13 pro-tein-coding genes(PCGs),two rRNA genes,22 tRNA genes,and one variable control region D ring.The base con-tent analysis reveals that the base composition of the carp has a high AT content bias.Furthermore,the 13 PCGs have many favorable codons,such as CGA(RSCU>3.506)and CCA(RSCU>2.208).Except for the tRNAGln,tRNAAla,tRNAAsn,tRNACys,tRNALeu(CUN),tRNATyr,tRNASer(UCN),tRNAPro,and ND6,the remaining genes are arranged on the H chain.The mitochondrial genome comparison between Hainan and Guangxi depicts that the similarity of the PCGs between them is between 96％and 100％.ND4,CO Ⅱ,and Cytb differ in their starting posi-tions,lengths,and starting codons in varying degrees.In the pound-to-pair comparison of 13 PCGs from C.carpio and Carassius auratus,which belong to the same genus,the similarity of the PCGs among the three species of Cy-prinus fish is between 85％and 99％.Constructing phylogenetic trees with the mitochondrial genome sequences of other cyprinids demonstrates the close relationship between C.acutidorsalis and C.carpio.The results will help us understand the relatives of different Cyprinidae and provide a reference for mitochondrial evolution analysis and germplasm resource research of C.acutidorsalis.关键词
尖鳍鲤(Cyprinus acutidorsalis)/线粒体基因组/系统发育/序列分析Key words
Cyprinus acutidorsalis/mitochondrial genome/phylogeny/sequence analysis分类
生物科学引用本文复制引用
蒙雨,宋一清,谢青颖,蔡杏伟,张清凤,董杨,李芳远,谢松光,申志新..海南万泉河口尖鳍鲤线粒体基因组测序与结构特征分析[J].海洋科学,2023,47(11):66-78,13.基金项目
海南省科技专项项目(ZDYF2023SHFZ101,ZDYF2021XDNY299) （ZDYF2023SHFZ101,ZDYF2021XDNY299）
海南省教育厅项目(Hnkyzc2022-4) （Hnkyzc2022-4）
海南大学科研启动基金项目(KYQD(ZR)21130)Hainan Province Science and Technology Special Fund,Nos.ZDYF2023SHFZ101,ZDYF2021XDNY299 （KYQD(ZR）
Project Supported by the Education Department of Hainan Province,No.Hnkyzc2022-4 （）
Scientific Research Fund of Hainan University for R&D,No.KYQD(ZR)21130 （ZR）
